High-yield production of short GpppA- and (7Me)GpppA-capped RNAs and HPLC-monitoring of methyltransfer reactions at the guanine-N7 and adenosine-2′O positions

Many eukaryotic and viral mRNAs, in which the first transcribed nucleotide is an adenosine, are decorated with a cap-1 structure, (7Me)G(5′)-ppp(5′)-A(2′OMe). The positive-sense RNA genomes of flaviviruses (Dengue, West Nile virus) for example show strict conservation of the adenosine. We set out to...

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Detalles Bibliográficos
Autores principales: Peyrane, F., Selisko, B., Decroly, E., Vasseur, J. J., Benarroch, D., Canard, B., Alvarez, K.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2007
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1851634/
https://www.ncbi.nlm.nih.gov/pubmed/17259217
http://dx.doi.org/10.1093/nar/gkl1119

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1851634/
https://www.ncbi.nlm.nih.gov/pubmed/17259217
http://dx.doi.org/10.1093/nar/gkl1119

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