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Sumoylation delays the ATF7 transcription factor subcellular localization and inhibits its transcriptional activity
Over the past few years, small ubiquitin-like modifier (SUMO) modification has emerged as an important regulator of diverse pathways and activities including protein localization and transcriptional regulation. We identified a consensus sumoylation motif (IKEE), located within the N-terminal activat...
Autores principales: | , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2007
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1851647/ https://www.ncbi.nlm.nih.gov/pubmed/17264123 http://dx.doi.org/10.1093/nar/gkl1168 |
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author | Hamard, Pierre-Jacques Boyer-Guittaut, Michaël Camuzeaux, Barbara Dujardin, Denis Hauss, Charlotte Oelgeschläger, Thomas Vigneron, Marc Kedinger, Claude Chatton, Bruno |
author_facet | Hamard, Pierre-Jacques Boyer-Guittaut, Michaël Camuzeaux, Barbara Dujardin, Denis Hauss, Charlotte Oelgeschläger, Thomas Vigneron, Marc Kedinger, Claude Chatton, Bruno |
author_sort | Hamard, Pierre-Jacques |
collection | PubMed |
description | Over the past few years, small ubiquitin-like modifier (SUMO) modification has emerged as an important regulator of diverse pathways and activities including protein localization and transcriptional regulation. We identified a consensus sumoylation motif (IKEE), located within the N-terminal activation domain of the ATF7 transcription factor and thus investigated the role of this modification. ATF7 is a ubiquitously expressed transcription factor, homologous to ATF2, that binds to CRE elements within specific promoters. This protein is able to heterodimerize with Jun or Fos proteins and its transcriptional activity is mediated by interaction with TAF12, a subunit of the general transcription factor TFIID. In the present article, we demonstrate that ATF7 is sumoylated in vitro (using RanBP2 as a E3-specific ligase) and in vivo. Moreover, we show that ATF7 sumoylation affects its intranuclear localization by delaying its entry into the nucleus. Furthermore, SUMO conjugation inhibits ATF7 transactivation activity by (i) impairing its association with TAF12 and (ii) blocking its binding-to-specific sequences within target promoters. |
format | Text |
id | pubmed-1851647 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2007 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-18516472007-04-26 Sumoylation delays the ATF7 transcription factor subcellular localization and inhibits its transcriptional activity Hamard, Pierre-Jacques Boyer-Guittaut, Michaël Camuzeaux, Barbara Dujardin, Denis Hauss, Charlotte Oelgeschläger, Thomas Vigneron, Marc Kedinger, Claude Chatton, Bruno Nucleic Acids Res Molecular Biology Over the past few years, small ubiquitin-like modifier (SUMO) modification has emerged as an important regulator of diverse pathways and activities including protein localization and transcriptional regulation. We identified a consensus sumoylation motif (IKEE), located within the N-terminal activation domain of the ATF7 transcription factor and thus investigated the role of this modification. ATF7 is a ubiquitously expressed transcription factor, homologous to ATF2, that binds to CRE elements within specific promoters. This protein is able to heterodimerize with Jun or Fos proteins and its transcriptional activity is mediated by interaction with TAF12, a subunit of the general transcription factor TFIID. In the present article, we demonstrate that ATF7 is sumoylated in vitro (using RanBP2 as a E3-specific ligase) and in vivo. Moreover, we show that ATF7 sumoylation affects its intranuclear localization by delaying its entry into the nucleus. Furthermore, SUMO conjugation inhibits ATF7 transactivation activity by (i) impairing its association with TAF12 and (ii) blocking its binding-to-specific sequences within target promoters. Oxford University Press 2007-02 2007-01-30 /pmc/articles/PMC1851647/ /pubmed/17264123 http://dx.doi.org/10.1093/nar/gkl1168 Text en © 2007 The Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Molecular Biology Hamard, Pierre-Jacques Boyer-Guittaut, Michaël Camuzeaux, Barbara Dujardin, Denis Hauss, Charlotte Oelgeschläger, Thomas Vigneron, Marc Kedinger, Claude Chatton, Bruno Sumoylation delays the ATF7 transcription factor subcellular localization and inhibits its transcriptional activity |
title | Sumoylation delays the ATF7 transcription factor subcellular localization and inhibits its transcriptional activity |
title_full | Sumoylation delays the ATF7 transcription factor subcellular localization and inhibits its transcriptional activity |
title_fullStr | Sumoylation delays the ATF7 transcription factor subcellular localization and inhibits its transcriptional activity |
title_full_unstemmed | Sumoylation delays the ATF7 transcription factor subcellular localization and inhibits its transcriptional activity |
title_short | Sumoylation delays the ATF7 transcription factor subcellular localization and inhibits its transcriptional activity |
title_sort | sumoylation delays the atf7 transcription factor subcellular localization and inhibits its transcriptional activity |
topic | Molecular Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1851647/ https://www.ncbi.nlm.nih.gov/pubmed/17264123 http://dx.doi.org/10.1093/nar/gkl1168 |
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