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Use of DNA melting simulation software for in silico diagnostic assay design: targeting regions with complex melting curves and confirmation by real-time PCR using intercalating dyes

BACKGROUND: DNA melting curve analysis using double-stranded DNA-specific dyes such as SYTO9 produce complex and reproducible melting profiles, resulting in the detection of multiple melting peaks from a single amplicon and allowing the discrimination of different species. We compare the melting cur...

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Detalles Bibliográficos
Autores principales:	Rasmussen, John P, Saint, Christopher P, Monis, Paul T
Formato:	Texto
Lenguaje:	English
Publicado:	BioMed Central 2007
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1851973/ https://www.ncbi.nlm.nih.gov/pubmed/17391531 http://dx.doi.org/10.1186/1471-2105-8-107

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