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Characterization of Escherichia coli MG1655 grown in a low-shear modeled microgravity environment

BACKGROUND: Extra-cellular shear force is an important environmental parameter that is significant both medically and in the space environment. Escherichia coli cells grown in a low-shear modeled microgravity (LSMMG) environment produced in a high aspect rotating vessel (HARV) were subjected to tran...

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Autores principales: Tucker, Don L, Ott, C Mark, Huff, Stephen, Fofanov, Yuriy, Pierson, Duane L, Willson, Richard C, Fox, George E
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1852313/
https://www.ncbi.nlm.nih.gov/pubmed/17343762
http://dx.doi.org/10.1186/1471-2180-7-15
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author Tucker, Don L
Ott, C Mark
Huff, Stephen
Fofanov, Yuriy
Pierson, Duane L
Willson, Richard C
Fox, George E
author_facet Tucker, Don L
Ott, C Mark
Huff, Stephen
Fofanov, Yuriy
Pierson, Duane L
Willson, Richard C
Fox, George E
author_sort Tucker, Don L
collection PubMed
description BACKGROUND: Extra-cellular shear force is an important environmental parameter that is significant both medically and in the space environment. Escherichia coli cells grown in a low-shear modeled microgravity (LSMMG) environment produced in a high aspect rotating vessel (HARV) were subjected to transcriptional and physiological analysis. RESULTS: Aerobic LSMMG cultures were grown in rich (LB) and minimal (MOPS + glucose) medium with a normal gravity vector HARV control. Reproducible changes in transcription were seen, but no specific LSMMG responsive genes were identified. Instead, absence of shear and a randomized gravity vector appears to cause local extra-cellular environmental changes, which elicit reproducible cellular responses. In minimal media, the majority of the significantly up- or down-regulated genes of known function were associated with the cell envelope. In rich medium, most LSMMG down-regulated genes were involved in translation. No observable changes in post-culture stress responses and antibiotic sensitivity were seen in cells immediately after exposure to LSMMG. Comparison with earlier studies of Salmonella enterica serovar Typhimurium conducted under similar growth conditions, revealed essentially no similarity in the genes that were significantly up- or down-regulated. CONCLUSION: Comparison of these results to previous studies suggests that different organisms may dramatically differ in their responses to medically significant low-shear and space environments. Depending on their specific response, some organisms, such as Salmonella, may become preadapted in a manner that predisposes them to increased virulence.
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spelling pubmed-18523132007-04-17 Characterization of Escherichia coli MG1655 grown in a low-shear modeled microgravity environment Tucker, Don L Ott, C Mark Huff, Stephen Fofanov, Yuriy Pierson, Duane L Willson, Richard C Fox, George E BMC Microbiol Research Article BACKGROUND: Extra-cellular shear force is an important environmental parameter that is significant both medically and in the space environment. Escherichia coli cells grown in a low-shear modeled microgravity (LSMMG) environment produced in a high aspect rotating vessel (HARV) were subjected to transcriptional and physiological analysis. RESULTS: Aerobic LSMMG cultures were grown in rich (LB) and minimal (MOPS + glucose) medium with a normal gravity vector HARV control. Reproducible changes in transcription were seen, but no specific LSMMG responsive genes were identified. Instead, absence of shear and a randomized gravity vector appears to cause local extra-cellular environmental changes, which elicit reproducible cellular responses. In minimal media, the majority of the significantly up- or down-regulated genes of known function were associated with the cell envelope. In rich medium, most LSMMG down-regulated genes were involved in translation. No observable changes in post-culture stress responses and antibiotic sensitivity were seen in cells immediately after exposure to LSMMG. Comparison with earlier studies of Salmonella enterica serovar Typhimurium conducted under similar growth conditions, revealed essentially no similarity in the genes that were significantly up- or down-regulated. CONCLUSION: Comparison of these results to previous studies suggests that different organisms may dramatically differ in their responses to medically significant low-shear and space environments. Depending on their specific response, some organisms, such as Salmonella, may become preadapted in a manner that predisposes them to increased virulence. BioMed Central 2007-03-07 /pmc/articles/PMC1852313/ /pubmed/17343762 http://dx.doi.org/10.1186/1471-2180-7-15 Text en Copyright © 2007 Tucker et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Tucker, Don L
Ott, C Mark
Huff, Stephen
Fofanov, Yuriy
Pierson, Duane L
Willson, Richard C
Fox, George E
Characterization of Escherichia coli MG1655 grown in a low-shear modeled microgravity environment
title Characterization of Escherichia coli MG1655 grown in a low-shear modeled microgravity environment
title_full Characterization of Escherichia coli MG1655 grown in a low-shear modeled microgravity environment
title_fullStr Characterization of Escherichia coli MG1655 grown in a low-shear modeled microgravity environment
title_full_unstemmed Characterization of Escherichia coli MG1655 grown in a low-shear modeled microgravity environment
title_short Characterization of Escherichia coli MG1655 grown in a low-shear modeled microgravity environment
title_sort characterization of escherichia coli mg1655 grown in a low-shear modeled microgravity environment
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1852313/
https://www.ncbi.nlm.nih.gov/pubmed/17343762
http://dx.doi.org/10.1186/1471-2180-7-15
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