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The uncoupling protein 1 gene, UCP1, is expressed in mammalian islet cells and associated with acute insulin response to glucose in African American families from the IRAS Family Study

BACKGROUND: Variants of uncoupling protein genes UCP1 and UCP2 have been associated with a range of traits. We wished to evaluate contributions of known UCP1 and UCP2 variants to metabolic traits in the Insulin Resistance and Atherosclerosis (IRAS) Family Study. METHODS: We genotyped five promoter o...

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Autores principales: Sale, Michèle M, Hsu, Fang-Chi, Palmer, Nicholette D, Gordon, Candace J, Keene, Keith L, Borgerink, Hermina M, Sharma, Arun J, Bergman, Richard N, Taylor, Kent D, Saad, Mohammed F, Norris, Jill M
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1852562/
https://www.ncbi.nlm.nih.gov/pubmed/17397545
http://dx.doi.org/10.1186/1472-6823-7-1
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author Sale, Michèle M
Hsu, Fang-Chi
Palmer, Nicholette D
Gordon, Candace J
Keene, Keith L
Borgerink, Hermina M
Sharma, Arun J
Bergman, Richard N
Taylor, Kent D
Saad, Mohammed F
Norris, Jill M
author_facet Sale, Michèle M
Hsu, Fang-Chi
Palmer, Nicholette D
Gordon, Candace J
Keene, Keith L
Borgerink, Hermina M
Sharma, Arun J
Bergman, Richard N
Taylor, Kent D
Saad, Mohammed F
Norris, Jill M
author_sort Sale, Michèle M
collection PubMed
description BACKGROUND: Variants of uncoupling protein genes UCP1 and UCP2 have been associated with a range of traits. We wished to evaluate contributions of known UCP1 and UCP2 variants to metabolic traits in the Insulin Resistance and Atherosclerosis (IRAS) Family Study. METHODS: We genotyped five promoter or coding single nucleotide polymorphisms (SNPs) in 239 African American (AA) participants and 583 Hispanic participants from San Antonio (SA) and San Luis Valley. Generalized estimating equations using a sandwich estimator of the variance and exchangeable correlation to account for familial correlation were computed for the test of genotypic association, and dominant, additive and recessive models. Tests were adjusted for age, gender and BMI (glucose homeostasis and lipid traits), or age and gender (obesity traits), and empirical P-values estimated using a gene dropping approach. RESULTS: UCP1 A-3826G was associated with AIR(g )in AA (P = 0.006) and approached significance in Hispanic families (P = 0.054); and with HDL-C levels in SA families (P = 0.0004). Although UCP1 expression is reported to be restricted to adipose tissue, RT-PCR indicated that UCP1 is expressed in human pancreas and MIN-6 cells, and immunohistochemistry demonstrated co-localization of UCP1 protein with insulin in human islets. UCP2 A55V was associated with waist circumference (P = 0.045) in AA, and BMI in SA (P = 0.018); and UCP2 G-866A with waist-to-hip ratio in AA (P = 0.016). CONCLUSION: This study suggests a functional variant of UCP1 contributes to the variance of AIR(g )in an AA population; the plausibility of this unexpected association is supported by the novel finding that UCP1 is expressed in islets.
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spelling pubmed-18525622007-04-18 The uncoupling protein 1 gene, UCP1, is expressed in mammalian islet cells and associated with acute insulin response to glucose in African American families from the IRAS Family Study Sale, Michèle M Hsu, Fang-Chi Palmer, Nicholette D Gordon, Candace J Keene, Keith L Borgerink, Hermina M Sharma, Arun J Bergman, Richard N Taylor, Kent D Saad, Mohammed F Norris, Jill M BMC Endocr Disord Research Article BACKGROUND: Variants of uncoupling protein genes UCP1 and UCP2 have been associated with a range of traits. We wished to evaluate contributions of known UCP1 and UCP2 variants to metabolic traits in the Insulin Resistance and Atherosclerosis (IRAS) Family Study. METHODS: We genotyped five promoter or coding single nucleotide polymorphisms (SNPs) in 239 African American (AA) participants and 583 Hispanic participants from San Antonio (SA) and San Luis Valley. Generalized estimating equations using a sandwich estimator of the variance and exchangeable correlation to account for familial correlation were computed for the test of genotypic association, and dominant, additive and recessive models. Tests were adjusted for age, gender and BMI (glucose homeostasis and lipid traits), or age and gender (obesity traits), and empirical P-values estimated using a gene dropping approach. RESULTS: UCP1 A-3826G was associated with AIR(g )in AA (P = 0.006) and approached significance in Hispanic families (P = 0.054); and with HDL-C levels in SA families (P = 0.0004). Although UCP1 expression is reported to be restricted to adipose tissue, RT-PCR indicated that UCP1 is expressed in human pancreas and MIN-6 cells, and immunohistochemistry demonstrated co-localization of UCP1 protein with insulin in human islets. UCP2 A55V was associated with waist circumference (P = 0.045) in AA, and BMI in SA (P = 0.018); and UCP2 G-866A with waist-to-hip ratio in AA (P = 0.016). CONCLUSION: This study suggests a functional variant of UCP1 contributes to the variance of AIR(g )in an AA population; the plausibility of this unexpected association is supported by the novel finding that UCP1 is expressed in islets. BioMed Central 2007-03-30 /pmc/articles/PMC1852562/ /pubmed/17397545 http://dx.doi.org/10.1186/1472-6823-7-1 Text en Copyright © 2007 Sale et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Sale, Michèle M
Hsu, Fang-Chi
Palmer, Nicholette D
Gordon, Candace J
Keene, Keith L
Borgerink, Hermina M
Sharma, Arun J
Bergman, Richard N
Taylor, Kent D
Saad, Mohammed F
Norris, Jill M
The uncoupling protein 1 gene, UCP1, is expressed in mammalian islet cells and associated with acute insulin response to glucose in African American families from the IRAS Family Study
title The uncoupling protein 1 gene, UCP1, is expressed in mammalian islet cells and associated with acute insulin response to glucose in African American families from the IRAS Family Study
title_full The uncoupling protein 1 gene, UCP1, is expressed in mammalian islet cells and associated with acute insulin response to glucose in African American families from the IRAS Family Study
title_fullStr The uncoupling protein 1 gene, UCP1, is expressed in mammalian islet cells and associated with acute insulin response to glucose in African American families from the IRAS Family Study
title_full_unstemmed The uncoupling protein 1 gene, UCP1, is expressed in mammalian islet cells and associated with acute insulin response to glucose in African American families from the IRAS Family Study
title_short The uncoupling protein 1 gene, UCP1, is expressed in mammalian islet cells and associated with acute insulin response to glucose in African American families from the IRAS Family Study
title_sort uncoupling protein 1 gene, ucp1, is expressed in mammalian islet cells and associated with acute insulin response to glucose in african american families from the iras family study
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1852562/
https://www.ncbi.nlm.nih.gov/pubmed/17397545
http://dx.doi.org/10.1186/1472-6823-7-1
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