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Cdc37 has distinct roles in protein kinase quality control that protect nascent chains from degradation and promote posttranslational maturation
Cdc37 is a molecular chaperone that functions with Hsp90 to promote protein kinase folding. Analysis of 65 Saccharomyces cerevisiae protein kinases (∼50% of the kinome) in a cdc37 mutant strain showed that 51 had decreased abundance compared with levels in the wild-type strain. Several lipid kinases...
Autores principales: | , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
2007
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1857360/ https://www.ncbi.nlm.nih.gov/pubmed/17242065 http://dx.doi.org/10.1083/jcb.200604106 |
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author | Mandal, Atin K. Lee, Paul Chen, Jennifer A. Nillegoda, Nadinath Heller, Alana DiStasio, Susan Oen, Handy Victor, Jacob Nair, Devi M. Brodsky, Jeffrey L. Caplan, Avrom J. |
author_facet | Mandal, Atin K. Lee, Paul Chen, Jennifer A. Nillegoda, Nadinath Heller, Alana DiStasio, Susan Oen, Handy Victor, Jacob Nair, Devi M. Brodsky, Jeffrey L. Caplan, Avrom J. |
author_sort | Mandal, Atin K. |
collection | PubMed |
description | Cdc37 is a molecular chaperone that functions with Hsp90 to promote protein kinase folding. Analysis of 65 Saccharomyces cerevisiae protein kinases (∼50% of the kinome) in a cdc37 mutant strain showed that 51 had decreased abundance compared with levels in the wild-type strain. Several lipid kinases also accumulated in reduced amounts in the cdc37 mutant strain. Results from our pulse-labeling studies showed that Cdc37 protects nascent kinase chains from rapid degradation shortly after synthesis. This degradation phenotype was suppressed when cdc37 mutant cells were grown at reduced temperatures, although this did not lead to a full restoration of kinase activity. We propose that Cdc37 functions at distinct steps in kinase biogenesis that involves protecting nascent chains from rapid degradation followed by its folding function in association with Hsp90. Our studies demonstrate that Cdc37 has a general role in kinome biogenesis. |
format | Text |
id | pubmed-1857360 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2007 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-18573602007-11-29 Cdc37 has distinct roles in protein kinase quality control that protect nascent chains from degradation and promote posttranslational maturation Mandal, Atin K. Lee, Paul Chen, Jennifer A. Nillegoda, Nadinath Heller, Alana DiStasio, Susan Oen, Handy Victor, Jacob Nair, Devi M. Brodsky, Jeffrey L. Caplan, Avrom J. J Cell Biol Research Articles Cdc37 is a molecular chaperone that functions with Hsp90 to promote protein kinase folding. Analysis of 65 Saccharomyces cerevisiae protein kinases (∼50% of the kinome) in a cdc37 mutant strain showed that 51 had decreased abundance compared with levels in the wild-type strain. Several lipid kinases also accumulated in reduced amounts in the cdc37 mutant strain. Results from our pulse-labeling studies showed that Cdc37 protects nascent kinase chains from rapid degradation shortly after synthesis. This degradation phenotype was suppressed when cdc37 mutant cells were grown at reduced temperatures, although this did not lead to a full restoration of kinase activity. We propose that Cdc37 functions at distinct steps in kinase biogenesis that involves protecting nascent chains from rapid degradation followed by its folding function in association with Hsp90. Our studies demonstrate that Cdc37 has a general role in kinome biogenesis. The Rockefeller University Press 2007-01-29 /pmc/articles/PMC1857360/ /pubmed/17242065 http://dx.doi.org/10.1083/jcb.200604106 Text en Copyright © 2007, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Research Articles Mandal, Atin K. Lee, Paul Chen, Jennifer A. Nillegoda, Nadinath Heller, Alana DiStasio, Susan Oen, Handy Victor, Jacob Nair, Devi M. Brodsky, Jeffrey L. Caplan, Avrom J. Cdc37 has distinct roles in protein kinase quality control that protect nascent chains from degradation and promote posttranslational maturation |
title | Cdc37 has distinct roles in protein kinase quality control that protect nascent chains from degradation and promote posttranslational maturation |
title_full | Cdc37 has distinct roles in protein kinase quality control that protect nascent chains from degradation and promote posttranslational maturation |
title_fullStr | Cdc37 has distinct roles in protein kinase quality control that protect nascent chains from degradation and promote posttranslational maturation |
title_full_unstemmed | Cdc37 has distinct roles in protein kinase quality control that protect nascent chains from degradation and promote posttranslational maturation |
title_short | Cdc37 has distinct roles in protein kinase quality control that protect nascent chains from degradation and promote posttranslational maturation |
title_sort | cdc37 has distinct roles in protein kinase quality control that protect nascent chains from degradation and promote posttranslational maturation |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1857360/ https://www.ncbi.nlm.nih.gov/pubmed/17242065 http://dx.doi.org/10.1083/jcb.200604106 |
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