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Human base excision repair complex is physically associated to DNA replication and cell cycle regulatory proteins
It has been hypothesized that a replication associated repair pathway operates on base damage and single strand breaks (SSB) at replication forks. In this study, we present the isolation from the nuclei of human cycling cells of a multiprotein complex containing most of the essential components of b...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
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Oxford University Press
2007
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1865045/ https://www.ncbi.nlm.nih.gov/pubmed/17289756 http://dx.doi.org/10.1093/nar/gkl1159 |
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author | Parlanti, Eleonora Locatelli, Giada Maga, Giovanni Dogliotti, Eugenia |
author_facet | Parlanti, Eleonora Locatelli, Giada Maga, Giovanni Dogliotti, Eugenia |
author_sort | Parlanti, Eleonora |
collection | PubMed |
description | It has been hypothesized that a replication associated repair pathway operates on base damage and single strand breaks (SSB) at replication forks. In this study, we present the isolation from the nuclei of human cycling cells of a multiprotein complex containing most of the essential components of base excision repair (BER)/SSBR, including APE1, UNG2, XRCC1 and POLβ, DNA PK, replicative POLα, δ and ɛ, DNA ligase 1 and cell cycle regulatory protein cyclin A. Co-immunoprecipitation revealed that in this complex DNA repair proteins are physically associated to cyclin A and to DNA replication proteins including MCM7. This complex is endowed with DNA polymerase and protein kinase activity and is able to perform BER of uracil and AP sites. This finding suggests that a preassembled DNA repair machinery is constitutively active in cycling cells and is ready to be recruited at base damage and breaks occurring at replication forks. |
format | Text |
id | pubmed-1865045 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2007 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-18650452007-05-22 Human base excision repair complex is physically associated to DNA replication and cell cycle regulatory proteins Parlanti, Eleonora Locatelli, Giada Maga, Giovanni Dogliotti, Eugenia Nucleic Acids Res Molecular Biology It has been hypothesized that a replication associated repair pathway operates on base damage and single strand breaks (SSB) at replication forks. In this study, we present the isolation from the nuclei of human cycling cells of a multiprotein complex containing most of the essential components of base excision repair (BER)/SSBR, including APE1, UNG2, XRCC1 and POLβ, DNA PK, replicative POLα, δ and ɛ, DNA ligase 1 and cell cycle regulatory protein cyclin A. Co-immunoprecipitation revealed that in this complex DNA repair proteins are physically associated to cyclin A and to DNA replication proteins including MCM7. This complex is endowed with DNA polymerase and protein kinase activity and is able to perform BER of uracil and AP sites. This finding suggests that a preassembled DNA repair machinery is constitutively active in cycling cells and is ready to be recruited at base damage and breaks occurring at replication forks. Oxford University Press 2007-03 2007-02-08 /pmc/articles/PMC1865045/ /pubmed/17289756 http://dx.doi.org/10.1093/nar/gkl1159 Text en © 2007 The Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Molecular Biology Parlanti, Eleonora Locatelli, Giada Maga, Giovanni Dogliotti, Eugenia Human base excision repair complex is physically associated to DNA replication and cell cycle regulatory proteins |
title | Human base excision repair complex is physically associated to DNA replication and cell cycle regulatory proteins |
title_full | Human base excision repair complex is physically associated to DNA replication and cell cycle regulatory proteins |
title_fullStr | Human base excision repair complex is physically associated to DNA replication and cell cycle regulatory proteins |
title_full_unstemmed | Human base excision repair complex is physically associated to DNA replication and cell cycle regulatory proteins |
title_short | Human base excision repair complex is physically associated to DNA replication and cell cycle regulatory proteins |
title_sort | human base excision repair complex is physically associated to dna replication and cell cycle regulatory proteins |
topic | Molecular Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1865045/ https://www.ncbi.nlm.nih.gov/pubmed/17289756 http://dx.doi.org/10.1093/nar/gkl1159 |
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