Cargando…

Biochemical and genetic analysis of RNA cap guanine-N2 methyltransferases from Giardia lamblia and Schizosaccharomyces pombe

RNA cap guanine-N2 methyltransferases such as Schizosaccharomyces pombe Tgs1 and Giardia lamblia Tgs2 catalyze methylation of the exocyclic N2 amine of 7-methylguanosine. Here we performed a mutational analysis of Giardia Tgs2, entailing an alanine scan of 17 residues within the minimal active domai...

Descripción completa

Detalles Bibliográficos
Autores principales:	Hausmann, Stéphane, Ramirez, Alejandro, Schneider, Susanne, Schwer, Beate, Shuman, Stewart
Formato:	Texto
Lenguaje:	English
Publicado:	Oxford University Press 2007
Materias:	Nucleic Acid Enzymes
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1865056/ https://www.ncbi.nlm.nih.gov/pubmed/17284461 http://dx.doi.org/10.1093/nar/gkl1150

Ejemplares similares

RNA surveillance by uridylation-dependent RNA decay in Schizosaccharomyces pombe
por: Chung, Christina Z, et al.
Publicado: (2019)

Identification and characterization of the mitochondrial RNA polymerase and transcription factor in the fission yeast Schizosaccharomyces pombe
por: Jiang, Hengyi, et al.
Publicado: (2011)

A second type of N7-guanine RNA cap methyltransferase in an unusual locus of a large RNA virus genome
por: Shannon, Ashleigh, et al.
Publicado: (2022)

Atomic structures of the RNA end-healing 5′-OH kinase and 2′,3′-cyclic phosphodiesterase domains of fungal tRNA ligase: conformational switches in the kinase upon binding of the GTP phosphate donor
por: Banerjee, Ankan, et al.
Publicado: (2019)

Pmt1, a Dnmt2 homolog in Schizosaccharomyces pombe, mediates tRNA methylation in response to nutrient signaling
por: Becker, Maria, et al.
Publicado: (2012)

DNA(3′)pp(5′)G de-capping activity of aprataxin: effect of cap nucleoside analogs and structural basis for guanosine recognition
por: Chauleau, Mathieu, et al.
Publicado: (2015)

NAD(+)-dependent synthesis of a 5′-phospho-ADP-ribosylated RNA/DNA cap by RNA 2′-phosphotransferase Tpt1
por: Munir, Annum, et al.
Publicado: (2018)

Functional characterization of a 48 kDa Trypanosoma brucei cap 2 RNA methyltransferase
por: Hall, Megan P., et al.
Publicado: (2006)

The methyltransferase domain of the Sudan ebolavirus L protein specifically targets internal adenosines of RNA substrates, in addition to the cap structure
por: Martin, Baptiste, et al.
Publicado: (2018)

The divergent eukaryote Trichomonas vaginalis has an m(7)G cap methyltransferase capable of a single N2 methylation
por: Simoes-Barbosa, Augusto, et al.
Publicado: (2008)

Mechanism of allosteric activation of human mRNA cap methyltransferase (RNMT) by RAM: insights from accelerated molecular dynamics simulations
por: Bueren-Calabuig, Juan A, et al.
Publicado: (2019)

Human DDX21 binds and unwinds RNA guanine quadruplexes
por: McRae, Ewan K.S., et al.
Publicado: (2017)

RNA triphosphatase is essential in Schizosaccharomyces pombe and Candida albicans
por: Pei, Yi, et al.
Publicado: (2001)

Structural and biochemical insight into the mechanism of dual CpG site binding and methylation by the DNMT3A DNA methyltransferase
por: Emperle, Max, et al.
Publicado: (2021)

Deinococcus radiodurans RNA ligase exemplifies a novel ligase clade with a distinctive N-terminal module that is important for 5′-PO(4) nick sealing and ligase adenylylation but dispensable for phosphodiester formation at an adenylylated nick
por: Raymond, Amy, et al.
Publicado: (2007)

Characterization of Agrobacterium tumefaciens DNA ligases C and D
por: Zhu, Hui, et al.
Publicado: (2007)

Characterization of the 2′,3′ cyclic phosphodiesterase activities of Clostridium thermocellum polynucleotide kinase-phosphatase and bacteriophage λ phosphatase
por: Keppetipola, Niroshika, et al.
Publicado: (2007)

Mycobacterium smegmatis SftH exemplifies a distinctive clade of superfamily II DNA-dependent ATPases with 3′ to 5′ translocase and helicase activities
por: Yakovleva, Lyudmila, et al.
Publicado: (2012)

Mechanism of RNA 2′,3′-cyclic phosphate end healing by T4 polynucleotide kinase–phosphatase
por: Das, Ushati, et al.
Publicado: (2013)

Kinetic mechanism and fidelity of nick sealing by Escherichia coli NAD(+)-dependent DNA ligase (LigA)
por: Chauleau, Mathieu, et al.
Publicado: (2016)

Mycobacterium smegmatis DinB2 misincorporates deoxyribonucleotides and ribonucleotides during templated synthesis and lesion bypass
por: Ordonez, Heather, et al.
Publicado: (2014)

Hyperthermophilic Aquifex aeolicus initiates primer synthesis on a limited set of trinucleotides comprised of cytosines and guanines
por: Larson, Marilynn A., et al.
Publicado: (2008)

The sequential 2′,3′-cyclic phosphodiesterase and 3′-phosphate/5′-OH ligation steps of the RtcB RNA splicing pathway are GTP-dependent
por: Chakravarty, Anupam K., et al.
Publicado: (2012)

Mutational analysis of the 5′-OH oligonucleotide phosphate acceptor site of T4 polynucleotide kinase
por: Wang, Li Kai, et al.
Publicado: (2010)

From general base to general acid catalysis in a sodium-specific DNAzyme by a guanine-to-adenine mutation
por: Ma, Lingzi, et al.
Publicado: (2019)

Structural insights to the metal specificity of an archaeal member of the LigD 3′-phosphoesterase DNA repair enzyme family
por: Das, Ushati, et al.
Publicado: (2012)

Mycobacterium smegmatis RqlH defines a novel clade of bacterial RecQ-like DNA helicases with ATP-dependent 3′–5′ translocase and duplex unwinding activities
por: Ordonez, Heather, et al.
Publicado: (2012)

Structural basis for the GTP specificity of the RNA kinase domain of fungal tRNA ligase
por: Remus, Barbara S., et al.
Publicado: (2017)

Mycobacterial DNA polymerase I: activities and crystal structures of the POL domain as apoenzyme and in complex with a DNA primer-template and of the full-length FEN/EXO–POL enzyme
por: Ghosh, Shreya, et al.
Publicado: (2020)

Novel non-specific DNA adenine methyltransferases
por: Drozdz, Marek, et al.
Publicado: (2012)

Structure and mechanism of the 2′,3′ phosphatase component of the bacterial Pnkp-Hen1 RNA repair system
por: Wang, Li Kai, et al.
Publicado: (2013)

Caveat mutator: alanine substitutions for conserved amino acids in RNA ligase elicit unexpected rearrangements of the active site for lysine adenylylation
por: Unciuleac, Mihaela-Carmen, et al.
Publicado: (2020)

The methyltransferase domain of dengue virus protein NS5 ensures efficient RNA synthesis initiation and elongation by the polymerase domain
por: Potisopon, Supanee, et al.
Publicado: (2014)

RNA modulation of the human DNA methyltransferase 3A
por: Holz-Schietinger, Celeste, et al.
Publicado: (2012)

Plasmid replication-associated single-strand-specific methyltransferases
por: Fomenkov, Alexey, et al.
Publicado: (2020)

A dual-functional priming-capping loop of rhabdoviral RNA polymerases directs terminal de novo initiation and capping intermediate formation
por: Ogino, Minako, et al.
Publicado: (2019)

Structure and two-metal mechanism of fungal tRNA ligase
por: Banerjee, Ankan, et al.
Publicado: (2019)

Pseudomonas putida MPE, a manganese-dependent endonuclease of the binuclear metallophosphoesterase superfamily, incises single-strand DNA in two orientations to yield a mixture of 3′-PO(4) and 3′-OH termini
por: Ghosh, Shreya, et al.
Publicado: (2020)

Crystal structure and mutational analysis of Mycobacterium smegmatis FenA highlight active site amino acids and three metal ions essential for flap endonuclease and 5′ exonuclease activities
por: Uson, Maria Loressa, et al.
Publicado: (2018)

The non-specific adenine DNA methyltransferase M.EcoGII
por: Murray, Iain A, et al.
Publicado: (2018)

Cannot write session to /tmp/vufind_sessions/sess_ti3rb7o8k4hdkour7trqgrmegu