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Rapid determination of the active fraction of DNA repair glycosylases: a novel fluorescence assay for trapped intermediates

Current methods to measure the fraction of active glycosylase molecules in a given enzyme preparation are slow and cumbersome. Here we report a novel assay for rapidly determining the active fraction based on molecular accessibility of a fluorescent DNA minor groove binder, 4′,6-diamidino-2-phenylin...

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Detalles Bibliográficos
Autores principales:	Blaisdell, Jeffrey O., Wallace, Susan S.
Formato:	Texto
Lenguaje:	English
Publicado:	Oxford University Press 2007
Materias:	Nucleic Acid Enzymes
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1865064/ https://www.ncbi.nlm.nih.gov/pubmed/17289752 http://dx.doi.org/10.1093/nar/gkm021

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