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Neurovirulence properties of recombinant vesicular stomatitis virus vectors in non-human primates

Although vesicular stomatitis virus (VSV) neurovirulence and pathogenicity in rodents have been well studied, little is known about VSV pathogenicity in non-human primates. To address this question, we measured VSV viremia, shedding, and neurovirulence in macaques. Following intranasal inoculation,...

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Autores principales: Johnson, J. Erik, Nasar, Farooq, Coleman, John W., Price, Roger E., Javadian, Ali, Draper, Kenneth, Lee, Margaret, Reilly, Patricia A., Clarke, David K., Hendry, R. Michael, Udem, Stephen A.
Formato: Texto
Lenguaje:English
Publicado: Elsevier Inc. 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1865117/
https://www.ncbi.nlm.nih.gov/pubmed/17098273
http://dx.doi.org/10.1016/j.virol.2006.10.026
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author Johnson, J. Erik
Nasar, Farooq
Coleman, John W.
Price, Roger E.
Javadian, Ali
Draper, Kenneth
Lee, Margaret
Reilly, Patricia A.
Clarke, David K.
Hendry, R. Michael
Udem, Stephen A.
author_facet Johnson, J. Erik
Nasar, Farooq
Coleman, John W.
Price, Roger E.
Javadian, Ali
Draper, Kenneth
Lee, Margaret
Reilly, Patricia A.
Clarke, David K.
Hendry, R. Michael
Udem, Stephen A.
author_sort Johnson, J. Erik
collection PubMed
description Although vesicular stomatitis virus (VSV) neurovirulence and pathogenicity in rodents have been well studied, little is known about VSV pathogenicity in non-human primates. To address this question, we measured VSV viremia, shedding, and neurovirulence in macaques. Following intranasal inoculation, macaques shed minimal recombinant VSV (rVSV) in nasal washes for 1 day post-inoculation; viremia was not detected. Following intranasal inoculation of macaques, wild type (wt) VSV, rVSV, and two rVSV-HIV vectors showed no evidence of spread to CNS tissues. However, macaques inoculated intrathalamically with wt VSV developed severe neurological disease. One of four macaques receiving rVSV developed clinical and histological signs similar to the wt group, while the remaining three macaques in this group and all of the macaques in the rVSV-HIV vector groups showed no clinical signs of disease and reduced severity of histopathology compared to the wt group. The implications of these findings for rVSV vaccine development are discussed.
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spelling pubmed-18651172008-03-30 Neurovirulence properties of recombinant vesicular stomatitis virus vectors in non-human primates Johnson, J. Erik Nasar, Farooq Coleman, John W. Price, Roger E. Javadian, Ali Draper, Kenneth Lee, Margaret Reilly, Patricia A. Clarke, David K. Hendry, R. Michael Udem, Stephen A. Virology Article Although vesicular stomatitis virus (VSV) neurovirulence and pathogenicity in rodents have been well studied, little is known about VSV pathogenicity in non-human primates. To address this question, we measured VSV viremia, shedding, and neurovirulence in macaques. Following intranasal inoculation, macaques shed minimal recombinant VSV (rVSV) in nasal washes for 1 day post-inoculation; viremia was not detected. Following intranasal inoculation of macaques, wild type (wt) VSV, rVSV, and two rVSV-HIV vectors showed no evidence of spread to CNS tissues. However, macaques inoculated intrathalamically with wt VSV developed severe neurological disease. One of four macaques receiving rVSV developed clinical and histological signs similar to the wt group, while the remaining three macaques in this group and all of the macaques in the rVSV-HIV vector groups showed no clinical signs of disease and reduced severity of histopathology compared to the wt group. The implications of these findings for rVSV vaccine development are discussed. Elsevier Inc. 2007-03-30 2006-11-13 /pmc/articles/PMC1865117/ /pubmed/17098273 http://dx.doi.org/10.1016/j.virol.2006.10.026 Text en Copyright © 2006 Elsevier Inc. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Johnson, J. Erik
Nasar, Farooq
Coleman, John W.
Price, Roger E.
Javadian, Ali
Draper, Kenneth
Lee, Margaret
Reilly, Patricia A.
Clarke, David K.
Hendry, R. Michael
Udem, Stephen A.
Neurovirulence properties of recombinant vesicular stomatitis virus vectors in non-human primates
title Neurovirulence properties of recombinant vesicular stomatitis virus vectors in non-human primates
title_full Neurovirulence properties of recombinant vesicular stomatitis virus vectors in non-human primates
title_fullStr Neurovirulence properties of recombinant vesicular stomatitis virus vectors in non-human primates
title_full_unstemmed Neurovirulence properties of recombinant vesicular stomatitis virus vectors in non-human primates
title_short Neurovirulence properties of recombinant vesicular stomatitis virus vectors in non-human primates
title_sort neurovirulence properties of recombinant vesicular stomatitis virus vectors in non-human primates
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1865117/
https://www.ncbi.nlm.nih.gov/pubmed/17098273
http://dx.doi.org/10.1016/j.virol.2006.10.026
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