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Resistance Exercise Reverses Aging in Human Skeletal Muscle
Human aging is associated with skeletal muscle atrophy and functional impairment (sarcopenia). Multiple lines of evidence suggest that mitochondrial dysfunction is a major contributor to sarcopenia. We evaluated whether healthy aging was associated with a transcriptional profile reflecting mitochond...
Autores principales: | , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2007
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1866181/ https://www.ncbi.nlm.nih.gov/pubmed/17520024 http://dx.doi.org/10.1371/journal.pone.0000465 |
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author | Melov, Simon Tarnopolsky, Mark A. Beckman, Kenneth Felkey, Krysta Hubbard, Alan |
author_facet | Melov, Simon Tarnopolsky, Mark A. Beckman, Kenneth Felkey, Krysta Hubbard, Alan |
author_sort | Melov, Simon |
collection | PubMed |
description | Human aging is associated with skeletal muscle atrophy and functional impairment (sarcopenia). Multiple lines of evidence suggest that mitochondrial dysfunction is a major contributor to sarcopenia. We evaluated whether healthy aging was associated with a transcriptional profile reflecting mitochondrial impairment and whether resistance exercise could reverse this signature to that approximating a younger physiological age. Skeletal muscle biopsies from healthy older (N = 25) and younger (N = 26) adult men and women were compared using gene expression profiling, and a subset of these were related to measurements of muscle strength. 14 of the older adults had muscle samples taken before and after a six-month resistance exercise-training program. Before exercise training, older adults were 59% weaker than younger, but after six months of training in older adults, strength improved significantly (P<0.001) such that they were only 38% lower than young adults. As a consequence of age, we found 596 genes differentially expressed using a false discovery rate cut-off of 5%. Prior to the exercise training, the transcriptome profile showed a dramatic enrichment of genes associated with mitochondrial function with age. However, following exercise training the transcriptional signature of aging was markedly reversed back to that of younger levels for most genes that were affected by both age and exercise. We conclude that healthy older adults show evidence of mitochondrial impairment and muscle weakness, but that this can be partially reversed at the phenotypic level, and substantially reversed at the transcriptome level, following six months of resistance exercise training. |
format | Text |
id | pubmed-1866181 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2007 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-18661812007-05-23 Resistance Exercise Reverses Aging in Human Skeletal Muscle Melov, Simon Tarnopolsky, Mark A. Beckman, Kenneth Felkey, Krysta Hubbard, Alan PLoS One Research Article Human aging is associated with skeletal muscle atrophy and functional impairment (sarcopenia). Multiple lines of evidence suggest that mitochondrial dysfunction is a major contributor to sarcopenia. We evaluated whether healthy aging was associated with a transcriptional profile reflecting mitochondrial impairment and whether resistance exercise could reverse this signature to that approximating a younger physiological age. Skeletal muscle biopsies from healthy older (N = 25) and younger (N = 26) adult men and women were compared using gene expression profiling, and a subset of these were related to measurements of muscle strength. 14 of the older adults had muscle samples taken before and after a six-month resistance exercise-training program. Before exercise training, older adults were 59% weaker than younger, but after six months of training in older adults, strength improved significantly (P<0.001) such that they were only 38% lower than young adults. As a consequence of age, we found 596 genes differentially expressed using a false discovery rate cut-off of 5%. Prior to the exercise training, the transcriptome profile showed a dramatic enrichment of genes associated with mitochondrial function with age. However, following exercise training the transcriptional signature of aging was markedly reversed back to that of younger levels for most genes that were affected by both age and exercise. We conclude that healthy older adults show evidence of mitochondrial impairment and muscle weakness, but that this can be partially reversed at the phenotypic level, and substantially reversed at the transcriptome level, following six months of resistance exercise training. Public Library of Science 2007-05-23 /pmc/articles/PMC1866181/ /pubmed/17520024 http://dx.doi.org/10.1371/journal.pone.0000465 Text en Melov et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Melov, Simon Tarnopolsky, Mark A. Beckman, Kenneth Felkey, Krysta Hubbard, Alan Resistance Exercise Reverses Aging in Human Skeletal Muscle |
title | Resistance Exercise Reverses Aging in Human Skeletal Muscle |
title_full | Resistance Exercise Reverses Aging in Human Skeletal Muscle |
title_fullStr | Resistance Exercise Reverses Aging in Human Skeletal Muscle |
title_full_unstemmed | Resistance Exercise Reverses Aging in Human Skeletal Muscle |
title_short | Resistance Exercise Reverses Aging in Human Skeletal Muscle |
title_sort | resistance exercise reverses aging in human skeletal muscle |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1866181/ https://www.ncbi.nlm.nih.gov/pubmed/17520024 http://dx.doi.org/10.1371/journal.pone.0000465 |
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