CAGE-TSSchip: promoter-based expression profiling using the 5'-leading label of capped transcripts

Cap analysis gene expression (CAGE) technology has revealed numerous transcription start sites (TSSs) in mammals and has suggested complex promoter-based patterns of regulation. We developed the CAGE-TSSchip to detect promoter-based transcriptional activity. The CAGE-TSSchip is a customized oligonuc...

Descripción completa

Detalles Bibliográficos
Autores principales: Katayama, Shintaro, Kanamori-Katayama, Mutsumi, Yamaguchi, Kazumi, Carninci, Piero, Hayashizaki, Yoshihide
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2007
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1868931/
http://dx.doi.org/10.1186/gb-2007-8-3-r42
Descripción
Sumario:Cap analysis gene expression (CAGE) technology has revealed numerous transcription start sites (TSSs) in mammals and has suggested complex promoter-based patterns of regulation. We developed the CAGE-TSSchip to detect promoter-based transcriptional activity. The CAGE-TSSchip is a customized oligonucleotide array that targets known TSSs identified by CAGE. A new labeling method, labeling capped transcripts from the 5'-end, had to be developed. The CAGE-TSSchip is accurate and sensitive, and represents the activity of each TSS.

Ejemplares similares