CAGE-TSSchip: promoter-based expression profiling using the 5'-leading label of capped transcripts

Cap analysis gene expression (CAGE) technology has revealed numerous transcription start sites (TSSs) in mammals and has suggested complex promoter-based patterns of regulation. We developed the CAGE-TSSchip to detect promoter-based transcriptional activity. The CAGE-TSSchip is a customized oligonuc...

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Detalles Bibliográficos
Autores principales: Katayama, Shintaro, Kanamori-Katayama, Mutsumi, Yamaguchi, Kazumi, Carninci, Piero, Hayashizaki, Yoshihide
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2007
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1868931/
http://dx.doi.org/10.1186/gb-2007-8-3-r42
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author Katayama, Shintaro
Kanamori-Katayama, Mutsumi
Yamaguchi, Kazumi
Carninci, Piero
Hayashizaki, Yoshihide
author_facet Katayama, Shintaro
Kanamori-Katayama, Mutsumi
Yamaguchi, Kazumi
Carninci, Piero
Hayashizaki, Yoshihide
author_sort Katayama, Shintaro
collection PubMed
description Cap analysis gene expression (CAGE) technology has revealed numerous transcription start sites (TSSs) in mammals and has suggested complex promoter-based patterns of regulation. We developed the CAGE-TSSchip to detect promoter-based transcriptional activity. The CAGE-TSSchip is a customized oligonucleotide array that targets known TSSs identified by CAGE. A new labeling method, labeling capped transcripts from the 5'-end, had to be developed. The CAGE-TSSchip is accurate and sensitive, and represents the activity of each TSS.
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spelling pubmed-18689312007-05-16 CAGE-TSSchip: promoter-based expression profiling using the 5'-leading label of capped transcripts Katayama, Shintaro Kanamori-Katayama, Mutsumi Yamaguchi, Kazumi Carninci, Piero Hayashizaki, Yoshihide Genome Biol Method Cap analysis gene expression (CAGE) technology has revealed numerous transcription start sites (TSSs) in mammals and has suggested complex promoter-based patterns of regulation. We developed the CAGE-TSSchip to detect promoter-based transcriptional activity. The CAGE-TSSchip is a customized oligonucleotide array that targets known TSSs identified by CAGE. A new labeling method, labeling capped transcripts from the 5'-end, had to be developed. The CAGE-TSSchip is accurate and sensitive, and represents the activity of each TSS. BioMed Central 2007 2007-03-26 /pmc/articles/PMC1868931/ http://dx.doi.org/10.1186/gb-2007-8-3-r42 Text en Copyright ©2007 Katayama et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Method
Katayama, Shintaro
Kanamori-Katayama, Mutsumi
Yamaguchi, Kazumi
Carninci, Piero
Hayashizaki, Yoshihide
CAGE-TSSchip: promoter-based expression profiling using the 5'-leading label of capped transcripts
title CAGE-TSSchip: promoter-based expression profiling using the 5'-leading label of capped transcripts
title_full CAGE-TSSchip: promoter-based expression profiling using the 5'-leading label of capped transcripts
title_fullStr CAGE-TSSchip: promoter-based expression profiling using the 5'-leading label of capped transcripts
title_full_unstemmed CAGE-TSSchip: promoter-based expression profiling using the 5'-leading label of capped transcripts
title_short CAGE-TSSchip: promoter-based expression profiling using the 5'-leading label of capped transcripts
title_sort cage-tsschip: promoter-based expression profiling using the 5'-leading label of capped transcripts
topic Method
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1868931/
http://dx.doi.org/10.1186/gb-2007-8-3-r42
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