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Proteome characterization of a human urothelial cell line resistant to the bladder carcinogen 4-aminobiphenyl
BACKGROUND: The aromatic amine 4-aminobiphenyl (4-ABP) is an environmental and occupational contaminant known to be a major etiological agent of human bladder cancer. 4-ABP metabolites are able to form DNA adducts that may induce mutations and initiate bladder carcinogenesis. Cells exposed to 4-ABP...
Autores principales: | , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2007
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1871571/ https://www.ncbi.nlm.nih.gov/pubmed/17477866 http://dx.doi.org/10.1186/1477-5956-5-6 |
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author | Pastorelli, Roberta Saletta, Federica Carpi, Donatella Campagna, Roberta dell'Osta, Carlo Schiarea, Silvia Vineis, Paolo Airoldi, Luisa Matullo, Giuseppe |
author_facet | Pastorelli, Roberta Saletta, Federica Carpi, Donatella Campagna, Roberta dell'Osta, Carlo Schiarea, Silvia Vineis, Paolo Airoldi, Luisa Matullo, Giuseppe |
author_sort | Pastorelli, Roberta |
collection | PubMed |
description | BACKGROUND: The aromatic amine 4-aminobiphenyl (4-ABP) is an environmental and occupational contaminant known to be a major etiological agent of human bladder cancer. 4-ABP metabolites are able to form DNA adducts that may induce mutations and initiate bladder carcinogenesis. Cells exposed to 4-ABP may develop resistance to the carcinogen. The aim of the present study was to detect and identify proteins whose expression is altered in the bladder carcinoma RT112 sub-lines selected for acquired resistance to 4-ABP, in order to disentangle the mechanisms. RESULTS: Differential proteome analysis of cell lysates showed an overall perturbation in cell metabolism and energy pathways in the 4-ABP-resistant human urothelial clones, with over-expression of membrane trafficking proteins such as annexin 2. The resistant clones had altered expression of many proteins linked directly (i.e. lamin A/C, programmed cell death 6 interacting protein) or indirectly (i.e. 94 kDa glucose-regulated protein, fatty acid-binding protein) to decreased apoptosis, suggesting that resistance to 4-ABP might be associated with low apoptotic activity. CONCLUSION: Our data provide evidence that deregulation of apoptosis and membrane trafficking proteins might be strongly implicated in the selection of carcinogen resistant cells. Some of these proteins might have potential as biomarkers of resistance and cancer risk. |
format | Text |
id | pubmed-1871571 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2007 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-18715712007-05-17 Proteome characterization of a human urothelial cell line resistant to the bladder carcinogen 4-aminobiphenyl Pastorelli, Roberta Saletta, Federica Carpi, Donatella Campagna, Roberta dell'Osta, Carlo Schiarea, Silvia Vineis, Paolo Airoldi, Luisa Matullo, Giuseppe Proteome Sci Research BACKGROUND: The aromatic amine 4-aminobiphenyl (4-ABP) is an environmental and occupational contaminant known to be a major etiological agent of human bladder cancer. 4-ABP metabolites are able to form DNA adducts that may induce mutations and initiate bladder carcinogenesis. Cells exposed to 4-ABP may develop resistance to the carcinogen. The aim of the present study was to detect and identify proteins whose expression is altered in the bladder carcinoma RT112 sub-lines selected for acquired resistance to 4-ABP, in order to disentangle the mechanisms. RESULTS: Differential proteome analysis of cell lysates showed an overall perturbation in cell metabolism and energy pathways in the 4-ABP-resistant human urothelial clones, with over-expression of membrane trafficking proteins such as annexin 2. The resistant clones had altered expression of many proteins linked directly (i.e. lamin A/C, programmed cell death 6 interacting protein) or indirectly (i.e. 94 kDa glucose-regulated protein, fatty acid-binding protein) to decreased apoptosis, suggesting that resistance to 4-ABP might be associated with low apoptotic activity. CONCLUSION: Our data provide evidence that deregulation of apoptosis and membrane trafficking proteins might be strongly implicated in the selection of carcinogen resistant cells. Some of these proteins might have potential as biomarkers of resistance and cancer risk. BioMed Central 2007-05-03 /pmc/articles/PMC1871571/ /pubmed/17477866 http://dx.doi.org/10.1186/1477-5956-5-6 Text en Copyright © 2007 Pastorelli et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Pastorelli, Roberta Saletta, Federica Carpi, Donatella Campagna, Roberta dell'Osta, Carlo Schiarea, Silvia Vineis, Paolo Airoldi, Luisa Matullo, Giuseppe Proteome characterization of a human urothelial cell line resistant to the bladder carcinogen 4-aminobiphenyl |
title | Proteome characterization of a human urothelial cell line resistant to the bladder carcinogen 4-aminobiphenyl |
title_full | Proteome characterization of a human urothelial cell line resistant to the bladder carcinogen 4-aminobiphenyl |
title_fullStr | Proteome characterization of a human urothelial cell line resistant to the bladder carcinogen 4-aminobiphenyl |
title_full_unstemmed | Proteome characterization of a human urothelial cell line resistant to the bladder carcinogen 4-aminobiphenyl |
title_short | Proteome characterization of a human urothelial cell line resistant to the bladder carcinogen 4-aminobiphenyl |
title_sort | proteome characterization of a human urothelial cell line resistant to the bladder carcinogen 4-aminobiphenyl |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1871571/ https://www.ncbi.nlm.nih.gov/pubmed/17477866 http://dx.doi.org/10.1186/1477-5956-5-6 |
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