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The use of global transcriptional analysis to reveal the biological and cellular events involved in distinct development phases of Trichophyton rubrum conidial germination

BACKGROUND: Conidia are considered to be the primary cause of infections by Trichophyton rubrum. RESULTS: We have developed a cDNA microarray containing 10250 ESTs to monitor the transcriptional strategy of conidial germination. A total of 1561 genes that had their expression levels specially altere...

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Autores principales: Liu, Tao, Zhang, Qian, Wang, Lingling, Yu, Lu, Leng, Wenchuan, Yang, Jian, Chen, Lihong, Peng, Junping, Ma, Li, Dong, Jie, Xu, Xingye, Xue, Ying, Zhu, Yafang, Zhang, Wenliang, Yang, Li, Li, Weijun, Sun, Lilian, Wan, Zhe, Ding, Guohui, Yu, Fudong, Tu, Kang, Qian, Ziliang, Li, Ruoyu, Shen, Yan, Li, Yixue, Jin, Qi
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1871584/
https://www.ncbi.nlm.nih.gov/pubmed/17428342
http://dx.doi.org/10.1186/1471-2164-8-100
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author Liu, Tao
Zhang, Qian
Wang, Lingling
Yu, Lu
Leng, Wenchuan
Yang, Jian
Chen, Lihong
Peng, Junping
Ma, Li
Dong, Jie
Xu, Xingye
Xue, Ying
Zhu, Yafang
Zhang, Wenliang
Yang, Li
Li, Weijun
Sun, Lilian
Wan, Zhe
Ding, Guohui
Yu, Fudong
Tu, Kang
Qian, Ziliang
Li, Ruoyu
Shen, Yan
Li, Yixue
Jin, Qi
author_facet Liu, Tao
Zhang, Qian
Wang, Lingling
Yu, Lu
Leng, Wenchuan
Yang, Jian
Chen, Lihong
Peng, Junping
Ma, Li
Dong, Jie
Xu, Xingye
Xue, Ying
Zhu, Yafang
Zhang, Wenliang
Yang, Li
Li, Weijun
Sun, Lilian
Wan, Zhe
Ding, Guohui
Yu, Fudong
Tu, Kang
Qian, Ziliang
Li, Ruoyu
Shen, Yan
Li, Yixue
Jin, Qi
author_sort Liu, Tao
collection PubMed
description BACKGROUND: Conidia are considered to be the primary cause of infections by Trichophyton rubrum. RESULTS: We have developed a cDNA microarray containing 10250 ESTs to monitor the transcriptional strategy of conidial germination. A total of 1561 genes that had their expression levels specially altered in the process were obtained and hierarchically clustered with respect to their expression profiles. By functional analysis, we provided a global view of an important biological system related to conidial germination, including characterization of the pattern of gene expression at sequential developmental phases, and changes of gene expression profiles corresponding to morphological transitions. We matched the EST sequences to GO terms in the Saccharomyces Genome Database (SGD). A number of homologues of Saccharomyces cerevisiae genes related to signalling pathways and some important cellular processes were found to be involved in T. rubrum germination. These genes and signalling pathways may play roles in distinct steps, such as activating conidial germination, maintenance of isotropic growth, establishment of cell polarity and morphological transitions. CONCLUSION: Our results may provide insights into molecular mechanisms of conidial germination at the cell level, and may enhance our understanding of regulation of gene expression related to the morphological construction of T. rubrum.
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spelling pubmed-18715842007-05-17 The use of global transcriptional analysis to reveal the biological and cellular events involved in distinct development phases of Trichophyton rubrum conidial germination Liu, Tao Zhang, Qian Wang, Lingling Yu, Lu Leng, Wenchuan Yang, Jian Chen, Lihong Peng, Junping Ma, Li Dong, Jie Xu, Xingye Xue, Ying Zhu, Yafang Zhang, Wenliang Yang, Li Li, Weijun Sun, Lilian Wan, Zhe Ding, Guohui Yu, Fudong Tu, Kang Qian, Ziliang Li, Ruoyu Shen, Yan Li, Yixue Jin, Qi BMC Genomics Research Article BACKGROUND: Conidia are considered to be the primary cause of infections by Trichophyton rubrum. RESULTS: We have developed a cDNA microarray containing 10250 ESTs to monitor the transcriptional strategy of conidial germination. A total of 1561 genes that had their expression levels specially altered in the process were obtained and hierarchically clustered with respect to their expression profiles. By functional analysis, we provided a global view of an important biological system related to conidial germination, including characterization of the pattern of gene expression at sequential developmental phases, and changes of gene expression profiles corresponding to morphological transitions. We matched the EST sequences to GO terms in the Saccharomyces Genome Database (SGD). A number of homologues of Saccharomyces cerevisiae genes related to signalling pathways and some important cellular processes were found to be involved in T. rubrum germination. These genes and signalling pathways may play roles in distinct steps, such as activating conidial germination, maintenance of isotropic growth, establishment of cell polarity and morphological transitions. CONCLUSION: Our results may provide insights into molecular mechanisms of conidial germination at the cell level, and may enhance our understanding of regulation of gene expression related to the morphological construction of T. rubrum. BioMed Central 2007-04-11 /pmc/articles/PMC1871584/ /pubmed/17428342 http://dx.doi.org/10.1186/1471-2164-8-100 Text en Copyright © 2007 Liu et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Liu, Tao
Zhang, Qian
Wang, Lingling
Yu, Lu
Leng, Wenchuan
Yang, Jian
Chen, Lihong
Peng, Junping
Ma, Li
Dong, Jie
Xu, Xingye
Xue, Ying
Zhu, Yafang
Zhang, Wenliang
Yang, Li
Li, Weijun
Sun, Lilian
Wan, Zhe
Ding, Guohui
Yu, Fudong
Tu, Kang
Qian, Ziliang
Li, Ruoyu
Shen, Yan
Li, Yixue
Jin, Qi
The use of global transcriptional analysis to reveal the biological and cellular events involved in distinct development phases of Trichophyton rubrum conidial germination
title The use of global transcriptional analysis to reveal the biological and cellular events involved in distinct development phases of Trichophyton rubrum conidial germination
title_full The use of global transcriptional analysis to reveal the biological and cellular events involved in distinct development phases of Trichophyton rubrum conidial germination
title_fullStr The use of global transcriptional analysis to reveal the biological and cellular events involved in distinct development phases of Trichophyton rubrum conidial germination
title_full_unstemmed The use of global transcriptional analysis to reveal the biological and cellular events involved in distinct development phases of Trichophyton rubrum conidial germination
title_short The use of global transcriptional analysis to reveal the biological and cellular events involved in distinct development phases of Trichophyton rubrum conidial germination
title_sort use of global transcriptional analysis to reveal the biological and cellular events involved in distinct development phases of trichophyton rubrum conidial germination
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1871584/
https://www.ncbi.nlm.nih.gov/pubmed/17428342
http://dx.doi.org/10.1186/1471-2164-8-100
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