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USER™ friendly DNA engineering and cloning method by uracil excision

Here we report a PCR-based DNA engineering technique for seamless assembly of recombinant molecules from multiple components. We create cloning vector and target molecules flanked with compatible single-stranded (ss) extensions. The vector contains a cassette with two inversely oriented nicking endo...

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Detalles Bibliográficos
Autores principales:	Bitinaite, Jurate, Rubino, Michelle, Varma, Kamini Hingorani, Schildkraut, Ira, Vaisvila, Romualdas, Vaiskunaite, Rita
Formato:	Texto
Lenguaje:	English
Publicado:	Oxford University Press 2007
Materias:	Nucleic Acid Enzymes
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1874603/ https://www.ncbi.nlm.nih.gov/pubmed/17341463 http://dx.doi.org/10.1093/nar/gkm041

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