Quantitative surface-enhanced resonance Raman scattering of phthalocyanine-labelled oligonucleotides

The evaluation of phthalocyanine labels for the surface-enhanced resonance Raman scattering (SERRS) detection of oligonucleotides is reported. Three phthalocyanine-labelled oligonucleotides were assessed, each containing a different metal centre. Detection limits for each labelled oligonucleotide we...

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Detalles Bibliográficos
Autores principales: Macaskill, A., Chernonosov, A. A., Koval, V. V., Lukyanets, E. A., Fedorova, O. S., Smith, W. E., Faulds, K., Graham, D.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2007
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1874604/
https://www.ncbi.nlm.nih.gov/pubmed/17289751
http://dx.doi.org/10.1093/nar/gkm042
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author Macaskill, A.
Chernonosov, A. A.
Koval, V. V.
Lukyanets, E. A.
Fedorova, O. S.
Smith, W. E.
Faulds, K.
Graham, D.
author_facet Macaskill, A.
Chernonosov, A. A.
Koval, V. V.
Lukyanets, E. A.
Fedorova, O. S.
Smith, W. E.
Faulds, K.
Graham, D.
author_sort Macaskill, A.
collection PubMed
description The evaluation of phthalocyanine labels for the surface-enhanced resonance Raman scattering (SERRS) detection of oligonucleotides is reported. Three phthalocyanine-labelled oligonucleotides were assessed, each containing a different metal centre. Detection limits for each labelled oligonucleotide were determined using two excitation frequencies where possible. Limits of detection as low as 2.8 × 10(−11) mol. dm(−3) were obtained which are comparable to standard fluorescently labelled probes used in previous SERRS studies. The identification of two phthalocyanine-labelled oligonucleotides without separation was also demonstrated indicating their suitability for multiplexing. This study extends the range of labels suitable for quantitative surface-enhanced resonance Raman scattering with silver nanoparticles and offers more flexibility and choice when considering SERRS for quantitative DNA detection.
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spelling pubmed-18746042007-05-23 Quantitative surface-enhanced resonance Raman scattering of phthalocyanine-labelled oligonucleotides Macaskill, A. Chernonosov, A. A. Koval, V. V. Lukyanets, E. A. Fedorova, O. S. Smith, W. E. Faulds, K. Graham, D. Nucleic Acids Res Methods Online The evaluation of phthalocyanine labels for the surface-enhanced resonance Raman scattering (SERRS) detection of oligonucleotides is reported. Three phthalocyanine-labelled oligonucleotides were assessed, each containing a different metal centre. Detection limits for each labelled oligonucleotide were determined using two excitation frequencies where possible. Limits of detection as low as 2.8 × 10(−11) mol. dm(−3) were obtained which are comparable to standard fluorescently labelled probes used in previous SERRS studies. The identification of two phthalocyanine-labelled oligonucleotides without separation was also demonstrated indicating their suitability for multiplexing. This study extends the range of labels suitable for quantitative surface-enhanced resonance Raman scattering with silver nanoparticles and offers more flexibility and choice when considering SERRS for quantitative DNA detection. Oxford University Press 2007-03 2007-02-08 /pmc/articles/PMC1874604/ /pubmed/17289751 http://dx.doi.org/10.1093/nar/gkm042 Text en © 2007 The Author(s) This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Methods Online
Macaskill, A.
Chernonosov, A. A.
Koval, V. V.
Lukyanets, E. A.
Fedorova, O. S.
Smith, W. E.
Faulds, K.
Graham, D.
Quantitative surface-enhanced resonance Raman scattering of phthalocyanine-labelled oligonucleotides
title Quantitative surface-enhanced resonance Raman scattering of phthalocyanine-labelled oligonucleotides
title_full Quantitative surface-enhanced resonance Raman scattering of phthalocyanine-labelled oligonucleotides
title_fullStr Quantitative surface-enhanced resonance Raman scattering of phthalocyanine-labelled oligonucleotides
title_full_unstemmed Quantitative surface-enhanced resonance Raman scattering of phthalocyanine-labelled oligonucleotides
title_short Quantitative surface-enhanced resonance Raman scattering of phthalocyanine-labelled oligonucleotides
title_sort quantitative surface-enhanced resonance raman scattering of phthalocyanine-labelled oligonucleotides
topic Methods Online
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1874604/
https://www.ncbi.nlm.nih.gov/pubmed/17289751
http://dx.doi.org/10.1093/nar/gkm042
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