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Site-directed transposon integration in human cells

The Sleeping Beauty (SB) transposon is a promising gene transfer vector that integrates nonspecifically into host cell genomes. Herein, we attempt to direct transposon integration into predetermined DNA sites by coupling a site-specific DNA-binding domain (DBD) to the SB transposase. We engineered f...

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Detalles Bibliográficos
Autores principales:	Yant, Stephen R., Huang, Yong, Akache, Bassel, Kay, Mark A.
Formato:	Texto
Lenguaje:	English
Publicado:	Oxford University Press 2007
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1874657/ https://www.ncbi.nlm.nih.gov/pubmed/17344320 http://dx.doi.org/10.1093/nar/gkm089

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