A Novel Linear Plasmid Mediates Flagellar Variation in Salmonella Typhi

Unlike the majority of Salmonella enterica serovars, Salmonella Typhi (S. Typhi), the etiological agent of human typhoid, is monophasic. S. Typhi normally harbours only the phase 1 flagellin gene (fliC), which encodes the H:d antigen. However, some S. Typhi strains found in Indonesia express an addi...

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Autores principales: Baker, Stephen, Hardy, Jonathan, Sanderson, Kenneth E, Quail, Michael, Goodhead, Ian, Kingsley, Robert A, Parkhill, Julian, Stocker, Bruce, Dougan, Gordon
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2007
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1876496/
https://www.ncbi.nlm.nih.gov/pubmed/17500588
http://dx.doi.org/10.1371/journal.ppat.0030059
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author Baker, Stephen
Hardy, Jonathan
Sanderson, Kenneth E
Quail, Michael
Goodhead, Ian
Kingsley, Robert A
Parkhill, Julian
Stocker, Bruce
Dougan, Gordon
author_facet Baker, Stephen
Hardy, Jonathan
Sanderson, Kenneth E
Quail, Michael
Goodhead, Ian
Kingsley, Robert A
Parkhill, Julian
Stocker, Bruce
Dougan, Gordon
author_sort Baker, Stephen
collection PubMed
description Unlike the majority of Salmonella enterica serovars, Salmonella Typhi (S. Typhi), the etiological agent of human typhoid, is monophasic. S. Typhi normally harbours only the phase 1 flagellin gene (fliC), which encodes the H:d antigen. However, some S. Typhi strains found in Indonesia express an additional flagellin antigen termed H:z66. Molecular analysis of H:z66+ S. Typhi revealed that the H:z66 flagellin structural gene (fljB(z66)) is encoded on a linear plasmid that we have named pBSSB1. The DNA sequence of pBSSB1 was determined to be just over 27 kbp, and was predicted to encode 33 coding sequences. To our knowledge, pBSSB1 is the first non-bacteriophage–related linear plasmid to be described in the Enterobacteriaceae.
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spelling pubmed-18764962007-05-24 A Novel Linear Plasmid Mediates Flagellar Variation in Salmonella Typhi Baker, Stephen Hardy, Jonathan Sanderson, Kenneth E Quail, Michael Goodhead, Ian Kingsley, Robert A Parkhill, Julian Stocker, Bruce Dougan, Gordon PLoS Pathog Research Article Unlike the majority of Salmonella enterica serovars, Salmonella Typhi (S. Typhi), the etiological agent of human typhoid, is monophasic. S. Typhi normally harbours only the phase 1 flagellin gene (fliC), which encodes the H:d antigen. However, some S. Typhi strains found in Indonesia express an additional flagellin antigen termed H:z66. Molecular analysis of H:z66+ S. Typhi revealed that the H:z66 flagellin structural gene (fljB(z66)) is encoded on a linear plasmid that we have named pBSSB1. The DNA sequence of pBSSB1 was determined to be just over 27 kbp, and was predicted to encode 33 coding sequences. To our knowledge, pBSSB1 is the first non-bacteriophage–related linear plasmid to be described in the Enterobacteriaceae. Public Library of Science 2007-05 2007-05-11 /pmc/articles/PMC1876496/ /pubmed/17500588 http://dx.doi.org/10.1371/journal.ppat.0030059 Text en © 2007 Baker et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Baker, Stephen
Hardy, Jonathan
Sanderson, Kenneth E
Quail, Michael
Goodhead, Ian
Kingsley, Robert A
Parkhill, Julian
Stocker, Bruce
Dougan, Gordon
A Novel Linear Plasmid Mediates Flagellar Variation in Salmonella Typhi
title A Novel Linear Plasmid Mediates Flagellar Variation in Salmonella Typhi
title_full A Novel Linear Plasmid Mediates Flagellar Variation in Salmonella Typhi
title_fullStr A Novel Linear Plasmid Mediates Flagellar Variation in Salmonella Typhi
title_full_unstemmed A Novel Linear Plasmid Mediates Flagellar Variation in Salmonella Typhi
title_short A Novel Linear Plasmid Mediates Flagellar Variation in Salmonella Typhi
title_sort novel linear plasmid mediates flagellar variation in salmonella typhi
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1876496/
https://www.ncbi.nlm.nih.gov/pubmed/17500588
http://dx.doi.org/10.1371/journal.ppat.0030059
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