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Mouse and rat BDNF gene structure and expression revisited
Brain-derived neurotrophic factor (BDNF) has important functions in the development of the nervous system and in brain plasticity-related processes such as memory, learning, and drug addiction. Despite the fact that the function and regulation of rodent BDNF gene expression have received close atten...
Autores principales: | , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Wiley Subscription Services, Inc., A Wiley Company
2007
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1878509/ https://www.ncbi.nlm.nih.gov/pubmed/17149751 http://dx.doi.org/10.1002/jnr.21139 |
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author | Aid, Tamara Kazantseva, Anna Piirsoo, Marko Palm, Kaia Timmusk, Tõnis |
author_facet | Aid, Tamara Kazantseva, Anna Piirsoo, Marko Palm, Kaia Timmusk, Tõnis |
author_sort | Aid, Tamara |
collection | PubMed |
description | Brain-derived neurotrophic factor (BDNF) has important functions in the development of the nervous system and in brain plasticity-related processes such as memory, learning, and drug addiction. Despite the fact that the function and regulation of rodent BDNF gene expression have received close attention during the last decade, knowledge of the structural organization of mouse and rat BDNF gene has remained incomplete. We have identified and characterized several mouse and rat BDNF transcripts containing novel 5′ untranslated exons and introduced a new numbering system for mouse and rat BDNF exons. According to our results both mouse and rat BDNF gene consist of eight 5′ untranslated exons and one protein coding 3′ exon. Transcription of the gene results in BDNF transcripts containing one of the eight 5′ exons spliced to the protein coding exon and in a transcript containing only 5′ extended protein coding exon. We also report the distinct tissue-specific expression profiles of each of the mouse and rat 5′ exon-specific transcripts in different brain regions and nonneural tissues. In addition, we show that kainic acid-induced seizures that lead to changes in cellular Ca(2+) levels as well as inhibition of DNA methylation and histone deacetylation contribute to the differential regulation of the expression of BDNF transcripts. Finally, we confirm that mouse and rat BDNF gene loci do not encode antisense mRNA transcripts, suggesting that mechanisms of regulation for rodent and human BDNF genes differ substantially. © 2006 Wiley-Liss, Inc. |
format | Text |
id | pubmed-1878509 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2007 |
publisher | Wiley Subscription Services, Inc., A Wiley Company |
record_format | MEDLINE/PubMed |
spelling | pubmed-18785092007-05-30 Mouse and rat BDNF gene structure and expression revisited Aid, Tamara Kazantseva, Anna Piirsoo, Marko Palm, Kaia Timmusk, Tõnis J Neurosci Res Research Article Brain-derived neurotrophic factor (BDNF) has important functions in the development of the nervous system and in brain plasticity-related processes such as memory, learning, and drug addiction. Despite the fact that the function and regulation of rodent BDNF gene expression have received close attention during the last decade, knowledge of the structural organization of mouse and rat BDNF gene has remained incomplete. We have identified and characterized several mouse and rat BDNF transcripts containing novel 5′ untranslated exons and introduced a new numbering system for mouse and rat BDNF exons. According to our results both mouse and rat BDNF gene consist of eight 5′ untranslated exons and one protein coding 3′ exon. Transcription of the gene results in BDNF transcripts containing one of the eight 5′ exons spliced to the protein coding exon and in a transcript containing only 5′ extended protein coding exon. We also report the distinct tissue-specific expression profiles of each of the mouse and rat 5′ exon-specific transcripts in different brain regions and nonneural tissues. In addition, we show that kainic acid-induced seizures that lead to changes in cellular Ca(2+) levels as well as inhibition of DNA methylation and histone deacetylation contribute to the differential regulation of the expression of BDNF transcripts. Finally, we confirm that mouse and rat BDNF gene loci do not encode antisense mRNA transcripts, suggesting that mechanisms of regulation for rodent and human BDNF genes differ substantially. © 2006 Wiley-Liss, Inc. Wiley Subscription Services, Inc., A Wiley Company 2007-02-15 2006-12-05 /pmc/articles/PMC1878509/ /pubmed/17149751 http://dx.doi.org/10.1002/jnr.21139 Text en Copyright © 2007 Wiley-Liss, Inc., A Wiley Company |
spellingShingle | Research Article Aid, Tamara Kazantseva, Anna Piirsoo, Marko Palm, Kaia Timmusk, Tõnis Mouse and rat BDNF gene structure and expression revisited |
title | Mouse and rat BDNF gene structure and expression revisited |
title_full | Mouse and rat BDNF gene structure and expression revisited |
title_fullStr | Mouse and rat BDNF gene structure and expression revisited |
title_full_unstemmed | Mouse and rat BDNF gene structure and expression revisited |
title_short | Mouse and rat BDNF gene structure and expression revisited |
title_sort | mouse and rat bdnf gene structure and expression revisited |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1878509/ https://www.ncbi.nlm.nih.gov/pubmed/17149751 http://dx.doi.org/10.1002/jnr.21139 |
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