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Comparison of approaches for rational siRNA design leading to a new efficient and transparent method

Current literature describes several methods for the design of efficient siRNAs with 19 perfectly matched base pairs and 2 nt overhangs. Using four independent databases totaling 3336 experimentally verified siRNAs, we compared how well several of these methods predict siRNA cleavage efficiency. Acc...

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Detalles Bibliográficos
Autores principales:	Matveeva, Olga, Nechipurenko, Yury, Rossi, Leo, Moore, Barry, Sætrom, Pål, Ogurtsov, Aleksey Y., Atkins, John F., Shabalina, Svetlana A.
Formato:	Texto
Lenguaje:	English
Publicado:	Oxford University Press 2007
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1885645/ https://www.ncbi.nlm.nih.gov/pubmed/17426130 http://dx.doi.org/10.1093/nar/gkm088

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