Cargando…

NXF2 is involved in cytoplasmic mRNA dynamics through interactions with motor proteins

Tap/NXF1, the founding member of the evolutionarily conserved NXF (Nuclear RNA export Factor) family of proteins, is required for the nuclear export of bulk poly(A)(+) RNAs. In mice, three additional NXF family genes (NXF2, NXF3, NXF7) have been identified and characterized to date. Cumulative data...

Descripción completa

Detalles Bibliográficos
Autores principales: Takano, Keizo, Miki, Takashi, Katahira, Jun, Yoneda, Yoshihiro
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2007
Materias:
RNA
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1885657/
https://www.ncbi.nlm.nih.gov/pubmed/17403691
http://dx.doi.org/10.1093/nar/gkm125
_version_ 1782133638939279360
author Takano, Keizo
Miki, Takashi
Katahira, Jun
Yoneda, Yoshihiro
author_facet Takano, Keizo
Miki, Takashi
Katahira, Jun
Yoneda, Yoshihiro
author_sort Takano, Keizo
collection PubMed
description Tap/NXF1, the founding member of the evolutionarily conserved NXF (Nuclear RNA export Factor) family of proteins, is required for the nuclear export of bulk poly(A)(+) RNAs. In mice, three additional NXF family genes (NXF2, NXF3, NXF7) have been identified and characterized to date. Cumulative data suggest that NXF family members play roles, not only in nuclear mRNA export, but also in various aspects of post-transcriptional mRNA metabolism. In order to better understand the functional role of NXF2, we searched for its binding partners by yeast two-hybrid screening and identified several cytoplasmic motor proteins, including KIF17. The interaction of NXF2 with KIF17, which was confirmed by GST pull-down and co-immunoprecipitation assays, is mediated by the N-terminal domain of NXF2, which is required for the punctate localization patterns in dendrites of primary neurons. We also found that the NXF2-containing dendritic granules, which were co-localized with KIF17, mRNA and Staufen1, a known component of neuronal mRNA granules, moved bidirectionally along dendrites in a microtubule-dependent manner. These results suggest that NXF2, a nucleo-cytoplasmic mRNA transporter, plays additional roles in the cytoplasmic localization of mRNAs through interactions with cytoplasmic motor proteins.
format Text
id pubmed-1885657
institution National Center for Biotechnology Information
language English
publishDate 2007
publisher Oxford University Press
record_format MEDLINE/PubMed
spelling pubmed-18856572007-06-07 NXF2 is involved in cytoplasmic mRNA dynamics through interactions with motor proteins Takano, Keizo Miki, Takashi Katahira, Jun Yoneda, Yoshihiro Nucleic Acids Res RNA Tap/NXF1, the founding member of the evolutionarily conserved NXF (Nuclear RNA export Factor) family of proteins, is required for the nuclear export of bulk poly(A)(+) RNAs. In mice, three additional NXF family genes (NXF2, NXF3, NXF7) have been identified and characterized to date. Cumulative data suggest that NXF family members play roles, not only in nuclear mRNA export, but also in various aspects of post-transcriptional mRNA metabolism. In order to better understand the functional role of NXF2, we searched for its binding partners by yeast two-hybrid screening and identified several cytoplasmic motor proteins, including KIF17. The interaction of NXF2 with KIF17, which was confirmed by GST pull-down and co-immunoprecipitation assays, is mediated by the N-terminal domain of NXF2, which is required for the punctate localization patterns in dendrites of primary neurons. We also found that the NXF2-containing dendritic granules, which were co-localized with KIF17, mRNA and Staufen1, a known component of neuronal mRNA granules, moved bidirectionally along dendrites in a microtubule-dependent manner. These results suggest that NXF2, a nucleo-cytoplasmic mRNA transporter, plays additional roles in the cytoplasmic localization of mRNAs through interactions with cytoplasmic motor proteins. Oxford University Press 2007-04 2007-04-01 /pmc/articles/PMC1885657/ /pubmed/17403691 http://dx.doi.org/10.1093/nar/gkm125 Text en © 2007 The Author(s) This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle RNA
Takano, Keizo
Miki, Takashi
Katahira, Jun
Yoneda, Yoshihiro
NXF2 is involved in cytoplasmic mRNA dynamics through interactions with motor proteins
title NXF2 is involved in cytoplasmic mRNA dynamics through interactions with motor proteins
title_full NXF2 is involved in cytoplasmic mRNA dynamics through interactions with motor proteins
title_fullStr NXF2 is involved in cytoplasmic mRNA dynamics through interactions with motor proteins
title_full_unstemmed NXF2 is involved in cytoplasmic mRNA dynamics through interactions with motor proteins
title_short NXF2 is involved in cytoplasmic mRNA dynamics through interactions with motor proteins
title_sort nxf2 is involved in cytoplasmic mrna dynamics through interactions with motor proteins
topic RNA
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1885657/
https://www.ncbi.nlm.nih.gov/pubmed/17403691
http://dx.doi.org/10.1093/nar/gkm125
work_keys_str_mv AT takanokeizo nxf2isinvolvedincytoplasmicmrnadynamicsthroughinteractionswithmotorproteins
AT mikitakashi nxf2isinvolvedincytoplasmicmrnadynamicsthroughinteractionswithmotorproteins
AT katahirajun nxf2isinvolvedincytoplasmicmrnadynamicsthroughinteractionswithmotorproteins
AT yonedayoshihiro nxf2isinvolvedincytoplasmicmrnadynamicsthroughinteractionswithmotorproteins