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Molecular Recognition of Lipid Antigens by T Cell Receptors

The T cell antigen receptor (TCR) mediates recognition of peptide antigens bound in the groove of major histocompatibility complex (MHC) molecules. This dual recognition is mediated by the complementarity-determining residue (CDR) loops of the α and β chains of a single TCR which contact exposed res...

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Autores principales: Grant, Ethan P., Degano, Massimo, Rosat, Jean-Pierre, Stenger, Steffen, Modlin, Robert L., Wilson, Ian A., Porcelli, Steven A., Brenner, Michael B.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1999
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1887682/
https://www.ncbi.nlm.nih.gov/pubmed/9874576
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author Grant, Ethan P.
Degano, Massimo
Rosat, Jean-Pierre
Stenger, Steffen
Modlin, Robert L.
Wilson, Ian A.
Porcelli, Steven A.
Brenner, Michael B.
author_facet Grant, Ethan P.
Degano, Massimo
Rosat, Jean-Pierre
Stenger, Steffen
Modlin, Robert L.
Wilson, Ian A.
Porcelli, Steven A.
Brenner, Michael B.
author_sort Grant, Ethan P.
collection PubMed
description The T cell antigen receptor (TCR) mediates recognition of peptide antigens bound in the groove of major histocompatibility complex (MHC) molecules. This dual recognition is mediated by the complementarity-determining residue (CDR) loops of the α and β chains of a single TCR which contact exposed residues of the peptide antigen and amino acids along the MHC α helices. The recent description of T cells that recognize hydrophobic microbial lipid antigens has challenged immunologists to explain, in molecular terms, the nature of this interaction. Structural studies on the murine CD1d1 molecule revealed an electrostatically neutral putative antigen-binding groove beneath the CD1 α helices. Here, we demonstrate that α/β TCRs, when transferred into TCR-deficient recipient cells, confer specificity for both the foreign lipid antigen and CD1 isoform. Sequence analysis of a panel of CD1-restricted, lipid-specific TCRs reveals the incorporation of template-independent N nucleotides that encode diverse sequences and frequent charged basic residues at the V(D)J junctions. These sequences permit a model for recognition in which the TCR CDR3 loops containing charged residues project between the CD1 α helices, contacting the lipid antigen hydrophilic head moieties as well as adjacent CD1 residues in a manner that explains antigen specificity and CD1 restriction.
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spelling pubmed-18876822008-04-16 Molecular Recognition of Lipid Antigens by T Cell Receptors Grant, Ethan P. Degano, Massimo Rosat, Jean-Pierre Stenger, Steffen Modlin, Robert L. Wilson, Ian A. Porcelli, Steven A. Brenner, Michael B. J Exp Med Articles The T cell antigen receptor (TCR) mediates recognition of peptide antigens bound in the groove of major histocompatibility complex (MHC) molecules. This dual recognition is mediated by the complementarity-determining residue (CDR) loops of the α and β chains of a single TCR which contact exposed residues of the peptide antigen and amino acids along the MHC α helices. The recent description of T cells that recognize hydrophobic microbial lipid antigens has challenged immunologists to explain, in molecular terms, the nature of this interaction. Structural studies on the murine CD1d1 molecule revealed an electrostatically neutral putative antigen-binding groove beneath the CD1 α helices. Here, we demonstrate that α/β TCRs, when transferred into TCR-deficient recipient cells, confer specificity for both the foreign lipid antigen and CD1 isoform. Sequence analysis of a panel of CD1-restricted, lipid-specific TCRs reveals the incorporation of template-independent N nucleotides that encode diverse sequences and frequent charged basic residues at the V(D)J junctions. These sequences permit a model for recognition in which the TCR CDR3 loops containing charged residues project between the CD1 α helices, contacting the lipid antigen hydrophilic head moieties as well as adjacent CD1 residues in a manner that explains antigen specificity and CD1 restriction. The Rockefeller University Press 1999-01-04 /pmc/articles/PMC1887682/ /pubmed/9874576 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Grant, Ethan P.
Degano, Massimo
Rosat, Jean-Pierre
Stenger, Steffen
Modlin, Robert L.
Wilson, Ian A.
Porcelli, Steven A.
Brenner, Michael B.
Molecular Recognition of Lipid Antigens by T Cell Receptors
title Molecular Recognition of Lipid Antigens by T Cell Receptors
title_full Molecular Recognition of Lipid Antigens by T Cell Receptors
title_fullStr Molecular Recognition of Lipid Antigens by T Cell Receptors
title_full_unstemmed Molecular Recognition of Lipid Antigens by T Cell Receptors
title_short Molecular Recognition of Lipid Antigens by T Cell Receptors
title_sort molecular recognition of lipid antigens by t cell receptors
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1887682/
https://www.ncbi.nlm.nih.gov/pubmed/9874576
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