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Pacemaker Synchronization of Electrically Coupled Rabbit Sinoatrial Node Cells

The effects of intercellular coupling conductance on the activity of two electrically coupled isolated rabbit sinoatrial nodal cells were investigated. A computer-controlled version of the “coupling clamp” technique was used in which isolated sinoatrial nodal cells, not physically in contact with ea...

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Autores principales: Verheijck, E. Etienne, Wilders, Ronald, Joyner, Ronald W., Golod, David A., Kumar, Rajiv, Jongsma, Habo J., Bouman, Lennart N., van Ginneken, Antoni C.G.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1998
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1887765/
https://www.ncbi.nlm.nih.gov/pubmed/9417138
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author Verheijck, E. Etienne
Wilders, Ronald
Joyner, Ronald W.
Golod, David A.
Kumar, Rajiv
Jongsma, Habo J.
Bouman, Lennart N.
van Ginneken, Antoni C.G.
author_facet Verheijck, E. Etienne
Wilders, Ronald
Joyner, Ronald W.
Golod, David A.
Kumar, Rajiv
Jongsma, Habo J.
Bouman, Lennart N.
van Ginneken, Antoni C.G.
author_sort Verheijck, E. Etienne
collection PubMed
description The effects of intercellular coupling conductance on the activity of two electrically coupled isolated rabbit sinoatrial nodal cells were investigated. A computer-controlled version of the “coupling clamp” technique was used in which isolated sinoatrial nodal cells, not physically in contact with each other, were electrically coupled at various values of ohmic coupling conductance, mimicking the effects of mutual interaction by electrical coupling through gap junctional channels. We demonstrate the existence of four types of electrical behavior of coupled spontaneously active cells. As the coupling conductance is progressively increased, the cells exhibit: (a) independent pacemaking at low coupling conductances, (b) complex dynamics of activity with mutual interactions, (c) entrainment of action potential frequency at a 1:1 ratio with different action potential waveforms, and (d) entrainment of action potentials at the same frequency of activation and virtually identical action potential waveforms. The critical value of coupling conductance required for 1:1 frequency entrainment was <0.5 nS in each of the five cell pairs studied. The common interbeat interval at a relatively high coupling conductance (10 nS), which is sufficient to produce entrainment of frequency and also identical action potential waveforms, is determined most by the intrinsically faster pacemaker cell and it can be predicted from the diastolic depolarization times of both cells. Evidence is provided that, at low coupling conductances, mutual pacemaker synchronization results mainly from the phase-resetting effects of the action potential of one cell on the depolarization phase of the other. At high coupling conductances, the tonic, diastolic interactions become more important.
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spelling pubmed-18877652008-04-22 Pacemaker Synchronization of Electrically Coupled Rabbit Sinoatrial Node Cells Verheijck, E. Etienne Wilders, Ronald Joyner, Ronald W. Golod, David A. Kumar, Rajiv Jongsma, Habo J. Bouman, Lennart N. van Ginneken, Antoni C.G. J Gen Physiol Article The effects of intercellular coupling conductance on the activity of two electrically coupled isolated rabbit sinoatrial nodal cells were investigated. A computer-controlled version of the “coupling clamp” technique was used in which isolated sinoatrial nodal cells, not physically in contact with each other, were electrically coupled at various values of ohmic coupling conductance, mimicking the effects of mutual interaction by electrical coupling through gap junctional channels. We demonstrate the existence of four types of electrical behavior of coupled spontaneously active cells. As the coupling conductance is progressively increased, the cells exhibit: (a) independent pacemaking at low coupling conductances, (b) complex dynamics of activity with mutual interactions, (c) entrainment of action potential frequency at a 1:1 ratio with different action potential waveforms, and (d) entrainment of action potentials at the same frequency of activation and virtually identical action potential waveforms. The critical value of coupling conductance required for 1:1 frequency entrainment was <0.5 nS in each of the five cell pairs studied. The common interbeat interval at a relatively high coupling conductance (10 nS), which is sufficient to produce entrainment of frequency and also identical action potential waveforms, is determined most by the intrinsically faster pacemaker cell and it can be predicted from the diastolic depolarization times of both cells. Evidence is provided that, at low coupling conductances, mutual pacemaker synchronization results mainly from the phase-resetting effects of the action potential of one cell on the depolarization phase of the other. At high coupling conductances, the tonic, diastolic interactions become more important. The Rockefeller University Press 1998-01-01 /pmc/articles/PMC1887765/ /pubmed/9417138 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Verheijck, E. Etienne
Wilders, Ronald
Joyner, Ronald W.
Golod, David A.
Kumar, Rajiv
Jongsma, Habo J.
Bouman, Lennart N.
van Ginneken, Antoni C.G.
Pacemaker Synchronization of Electrically Coupled Rabbit Sinoatrial Node Cells
title Pacemaker Synchronization of Electrically Coupled Rabbit Sinoatrial Node Cells
title_full Pacemaker Synchronization of Electrically Coupled Rabbit Sinoatrial Node Cells
title_fullStr Pacemaker Synchronization of Electrically Coupled Rabbit Sinoatrial Node Cells
title_full_unstemmed Pacemaker Synchronization of Electrically Coupled Rabbit Sinoatrial Node Cells
title_short Pacemaker Synchronization of Electrically Coupled Rabbit Sinoatrial Node Cells
title_sort pacemaker synchronization of electrically coupled rabbit sinoatrial node cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1887765/
https://www.ncbi.nlm.nih.gov/pubmed/9417138
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