Base-specific spin-labeling of RNA for structure determination

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To facilitate the measurement of intramolecular distances in solvated RNA systems, a combination of spin-labeling, electron paramagnetic resonance (EPR), and molecular dynamics (MD) simulation is presented. The fairly rigid spin label 2,2,5,5-tetramethyl-pyrrolin-1-yloxyl-3-acetylene (TPA) was base...

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Detalles Bibliográficos
Autores principales: Piton, Nelly, Mu, Yuguang, Stock, Gerhard, Prisner, Thomas F., Schiemann, Olav, Engels, Joachim W.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2007
Materias:
RNA
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1891445/
https://www.ncbi.nlm.nih.gov/pubmed/17452362
http://dx.doi.org/10.1093/nar/gkm169

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