Expression, limited proteolysis and preliminary crystallographic analysis of IpaD, a component of the Shigella flexneri type III secretion system

IpaD, the putative needle-tip protein of the Shigella flexneri type III secretion system, has been overexpressed and purified. Crystals were grown of the native protein in space group P2(1)2(1)2(1), with unit-cell parameters a = 55.9, b = 100.7, c = 112.0 Å, and data were collected to 2.9 Å resoluti...

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Detalles Bibliográficos
Autores principales: Johnson, Steven, Roversi, Pietro, Espina, Marianela, Deane, Janet E., Birket, Susan, Picking, William D., Blocker, Ariel, Picking, Wendy L., Lea, Susan M.
Formato: Texto
Lenguaje:English
Publicado: International Union of Crystallography 2006
Materias:
Crystallization Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1894744/
https://www.ncbi.nlm.nih.gov/pubmed/16946465
http://dx.doi.org/10.1107/S1744309106027047

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