Microtitre plate-based antibacterial assay incorporating resazurin as an indicator of cell growth, and its application in the in vitro antibacterial screening of phytochemicals

The resazurin assay utilising microtitre-plate, described by Drummond and Waigh in 2000, has been modified to achieve more accuracy in the determination of the minimum inhibitory concentration (MIC) values of natural products, including crude extracts, chromatographic fractions or purified compounds...

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Detalles Bibliográficos
Autores principales: Sarker, Satyajit D., Nahar, Lutfun, Kumarasamy, Yashodharan
Formato: Texto
Lenguaje:English
Publicado: Academic Press 2007
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1895922/
https://www.ncbi.nlm.nih.gov/pubmed/17560319
http://dx.doi.org/10.1016/j.ymeth.2007.01.006
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author Sarker, Satyajit D.
Nahar, Lutfun
Kumarasamy, Yashodharan
author_facet Sarker, Satyajit D.
Nahar, Lutfun
Kumarasamy, Yashodharan
author_sort Sarker, Satyajit D.
collection PubMed
description The resazurin assay utilising microtitre-plate, described by Drummond and Waigh in 2000, has been modified to achieve more accuracy in the determination of the minimum inhibitory concentration (MIC) values of natural products, including crude extracts, chromatographic fractions or purified compounds against various bacterial strains. This modified resazurin method is simple, sensitive, rapid, robust and reliable, and could be used successfully to assess antibacterial properties of natural products.
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spelling pubmed-18959222007-06-29 Microtitre plate-based antibacterial assay incorporating resazurin as an indicator of cell growth, and its application in the in vitro antibacterial screening of phytochemicals Sarker, Satyajit D. Nahar, Lutfun Kumarasamy, Yashodharan Methods Article The resazurin assay utilising microtitre-plate, described by Drummond and Waigh in 2000, has been modified to achieve more accuracy in the determination of the minimum inhibitory concentration (MIC) values of natural products, including crude extracts, chromatographic fractions or purified compounds against various bacterial strains. This modified resazurin method is simple, sensitive, rapid, robust and reliable, and could be used successfully to assess antibacterial properties of natural products. Academic Press 2007-08 /pmc/articles/PMC1895922/ /pubmed/17560319 http://dx.doi.org/10.1016/j.ymeth.2007.01.006 Text en . https://creativecommons.org/licenses/by-nc-nd/3.0/ Open Access under CC BY-NC-ND 3.0 (https://creativecommons.org/licenses/by-nc-nd/3.0/) license
spellingShingle Article
Sarker, Satyajit D.
Nahar, Lutfun
Kumarasamy, Yashodharan
Microtitre plate-based antibacterial assay incorporating resazurin as an indicator of cell growth, and its application in the in vitro antibacterial screening of phytochemicals
title Microtitre plate-based antibacterial assay incorporating resazurin as an indicator of cell growth, and its application in the in vitro antibacterial screening of phytochemicals
title_full Microtitre plate-based antibacterial assay incorporating resazurin as an indicator of cell growth, and its application in the in vitro antibacterial screening of phytochemicals
title_fullStr Microtitre plate-based antibacterial assay incorporating resazurin as an indicator of cell growth, and its application in the in vitro antibacterial screening of phytochemicals
title_full_unstemmed Microtitre plate-based antibacterial assay incorporating resazurin as an indicator of cell growth, and its application in the in vitro antibacterial screening of phytochemicals
title_short Microtitre plate-based antibacterial assay incorporating resazurin as an indicator of cell growth, and its application in the in vitro antibacterial screening of phytochemicals
title_sort microtitre plate-based antibacterial assay incorporating resazurin as an indicator of cell growth, and its application in the in vitro antibacterial screening of phytochemicals
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1895922/
https://www.ncbi.nlm.nih.gov/pubmed/17560319
http://dx.doi.org/10.1016/j.ymeth.2007.01.006
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