A major zebrafish polymorphism resource for genetic mapping

We have identified 645,088 candidate polymorphisms in zebrafish and observe a single nucleotide polymorphism (SNP) validation rate of 71% to 86%, improving with polymorphism confidence score. Variant sites are non-random, with an excess of specific novel T- and A-rich motifs. We positioned half of t...

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Detalles Bibliográficos
Autores principales: Bradley, Kevin M, Elmore, J Bradford, Breyer, Joan P, Yaspan, Brian L, Jessen, Jason R, Knapik, Ela W, Smith, Jeffrey R
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2007
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1896001/
https://www.ncbi.nlm.nih.gov/pubmed/17428331
http://dx.doi.org/10.1186/gb-2007-8-4-r55
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author Bradley, Kevin M
Elmore, J Bradford
Breyer, Joan P
Yaspan, Brian L
Jessen, Jason R
Knapik, Ela W
Smith, Jeffrey R
author_facet Bradley, Kevin M
Elmore, J Bradford
Breyer, Joan P
Yaspan, Brian L
Jessen, Jason R
Knapik, Ela W
Smith, Jeffrey R
author_sort Bradley, Kevin M
collection PubMed
description We have identified 645,088 candidate polymorphisms in zebrafish and observe a single nucleotide polymorphism (SNP) validation rate of 71% to 86%, improving with polymorphism confidence score. Variant sites are non-random, with an excess of specific novel T- and A-rich motifs. We positioned half of the polymorphisms on zebrafish genetic and physical maps as a resource for positional cloning. We further demonstrate bulked segregant analysis using the anchored SNPs as a method for high-throughput genetic mapping in zebrafish.
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spelling pubmed-18960012007-06-22 A major zebrafish polymorphism resource for genetic mapping Bradley, Kevin M Elmore, J Bradford Breyer, Joan P Yaspan, Brian L Jessen, Jason R Knapik, Ela W Smith, Jeffrey R Genome Biol Method We have identified 645,088 candidate polymorphisms in zebrafish and observe a single nucleotide polymorphism (SNP) validation rate of 71% to 86%, improving with polymorphism confidence score. Variant sites are non-random, with an excess of specific novel T- and A-rich motifs. We positioned half of the polymorphisms on zebrafish genetic and physical maps as a resource for positional cloning. We further demonstrate bulked segregant analysis using the anchored SNPs as a method for high-throughput genetic mapping in zebrafish. BioMed Central 2007 2007-04-11 /pmc/articles/PMC1896001/ /pubmed/17428331 http://dx.doi.org/10.1186/gb-2007-8-4-r55 Text en Copyright © 2007 Bradley et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Method
Bradley, Kevin M
Elmore, J Bradford
Breyer, Joan P
Yaspan, Brian L
Jessen, Jason R
Knapik, Ela W
Smith, Jeffrey R
A major zebrafish polymorphism resource for genetic mapping
title A major zebrafish polymorphism resource for genetic mapping
title_full A major zebrafish polymorphism resource for genetic mapping
title_fullStr A major zebrafish polymorphism resource for genetic mapping
title_full_unstemmed A major zebrafish polymorphism resource for genetic mapping
title_short A major zebrafish polymorphism resource for genetic mapping
title_sort major zebrafish polymorphism resource for genetic mapping
topic Method
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1896001/
https://www.ncbi.nlm.nih.gov/pubmed/17428331
http://dx.doi.org/10.1186/gb-2007-8-4-r55
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