A semi-automated high-throughput approach to the generation of transposon insertion mutants in the nematode Caenorhabditis elegans

The generation of a large collection of defined transposon insertion mutants is of general interest to the Caenorhabditis elegans research community and has been supported by the European Union. We describe here a semi-automated high-throughput method for mutant production and screening, using the h...

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Autores principales: Duverger, Yohann, Belougne, Jérôme, Scaglione, Sarah, Brandli, Dominique, Beclin, Christophe, Ewbank, Jonathan J.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2007
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1903375/
https://www.ncbi.nlm.nih.gov/pubmed/17164286
http://dx.doi.org/10.1093/nar/gkl1046
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author Duverger, Yohann
Belougne, Jérôme
Scaglione, Sarah
Brandli, Dominique
Beclin, Christophe
Ewbank, Jonathan J.
author_facet Duverger, Yohann
Belougne, Jérôme
Scaglione, Sarah
Brandli, Dominique
Beclin, Christophe
Ewbank, Jonathan J.
author_sort Duverger, Yohann
collection PubMed
description The generation of a large collection of defined transposon insertion mutants is of general interest to the Caenorhabditis elegans research community and has been supported by the European Union. We describe here a semi-automated high-throughput method for mutant production and screening, using the heterologous transposon Mos1. The procedure allows routine culture of several thousand independent nematode strains in parallel for multiple generations before stereotyped molecular analyses. Using this method, we have already generated >17 500 individual strains carrying Mos1 insertions. It could be easily adapted to forward and reverse genetic screens and may influence researchers faced with making a choice of model organism.
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spelling pubmed-19033752007-06-28 A semi-automated high-throughput approach to the generation of transposon insertion mutants in the nematode Caenorhabditis elegans Duverger, Yohann Belougne, Jérôme Scaglione, Sarah Brandli, Dominique Beclin, Christophe Ewbank, Jonathan J. Nucleic Acids Res Methods Online The generation of a large collection of defined transposon insertion mutants is of general interest to the Caenorhabditis elegans research community and has been supported by the European Union. We describe here a semi-automated high-throughput method for mutant production and screening, using the heterologous transposon Mos1. The procedure allows routine culture of several thousand independent nematode strains in parallel for multiple generations before stereotyped molecular analyses. Using this method, we have already generated >17 500 individual strains carrying Mos1 insertions. It could be easily adapted to forward and reverse genetic screens and may influence researchers faced with making a choice of model organism. Oxford University Press 2007-01 2006-12-12 /pmc/articles/PMC1903375/ /pubmed/17164286 http://dx.doi.org/10.1093/nar/gkl1046 Text en © 2006 The Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Methods Online
Duverger, Yohann
Belougne, Jérôme
Scaglione, Sarah
Brandli, Dominique
Beclin, Christophe
Ewbank, Jonathan J.
A semi-automated high-throughput approach to the generation of transposon insertion mutants in the nematode Caenorhabditis elegans
title A semi-automated high-throughput approach to the generation of transposon insertion mutants in the nematode Caenorhabditis elegans
title_full A semi-automated high-throughput approach to the generation of transposon insertion mutants in the nematode Caenorhabditis elegans
title_fullStr A semi-automated high-throughput approach to the generation of transposon insertion mutants in the nematode Caenorhabditis elegans
title_full_unstemmed A semi-automated high-throughput approach to the generation of transposon insertion mutants in the nematode Caenorhabditis elegans
title_short A semi-automated high-throughput approach to the generation of transposon insertion mutants in the nematode Caenorhabditis elegans
title_sort semi-automated high-throughput approach to the generation of transposon insertion mutants in the nematode caenorhabditis elegans
topic Methods Online
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1903375/
https://www.ncbi.nlm.nih.gov/pubmed/17164286
http://dx.doi.org/10.1093/nar/gkl1046
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