Protein–protein interaction analysis by C-terminally specific fluorescence labeling and fluorescence cross-correlation spectroscopy

Here, we describe novel puromycin derivatives conjugated with iminobiotin and a fluorescent dye that can be linked covalently to the C-terminus of full-length proteins during cell-free translation. The iminobiotin-labeled proteins can be highly purified by affinity purification with streptavidin bea...

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Detalles Bibliográficos
Autores principales: Oyama, Rieko, Takashima, Hideaki, Yonezawa, Masato, Doi, Nobuhide, Miyamoto-Sato, Etsuko, Kinjo, Masataka, Yanagawa, Hiroshi
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2006
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1904107/
https://www.ncbi.nlm.nih.gov/pubmed/16914444
http://dx.doi.org/10.1093/nar/gkl477

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1904107/
https://www.ncbi.nlm.nih.gov/pubmed/16914444
http://dx.doi.org/10.1093/nar/gkl477

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