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Differential in vitro inhibitory activity against HIV-1 of alpha-(1-3)- and alpha-(1-6)-D-mannose specific plant lectins : Implication for microbicide development

BACKGROUND: Plant lectins such as Galanthus nivalis agglutinin (GNA) and Hippeastrum hybrid agglutinin (HHA) are natural proteins able to link mannose residues, and therefore inhibit HIV-target cell interactions. Plant lectins are candidate for microbicide development. OBJECTIVE: To evaluate the act...

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Autores principales: Saïdi, Hela, Nasreddine, Nadine, Jenabian, Mohammad-Ali, Lecerf, Maxime, Schols, Dominique, Krief, Corinne, Balzarini, Jan, Bélec, Laurent
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1904181/
https://www.ncbi.nlm.nih.gov/pubmed/17565674
http://dx.doi.org/10.1186/1479-5876-5-28
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author Saïdi, Hela
Nasreddine, Nadine
Jenabian, Mohammad-Ali
Lecerf, Maxime
Schols, Dominique
Krief, Corinne
Balzarini, Jan
Bélec, Laurent
author_facet Saïdi, Hela
Nasreddine, Nadine
Jenabian, Mohammad-Ali
Lecerf, Maxime
Schols, Dominique
Krief, Corinne
Balzarini, Jan
Bélec, Laurent
author_sort Saïdi, Hela
collection PubMed
description BACKGROUND: Plant lectins such as Galanthus nivalis agglutinin (GNA) and Hippeastrum hybrid agglutinin (HHA) are natural proteins able to link mannose residues, and therefore inhibit HIV-target cell interactions. Plant lectins are candidate for microbicide development. OBJECTIVE: To evaluate the activity against HIV of the mannose-specific plant lectins HHA and GNA at the cellular membrane level of epithelial cells and monocyte-derived dendritic cells (MDDC), two potential target cells of HIV at the genital mucosal level. METHODS: The inhibitory effects of HHA and GNA were evaluated on HIV adsorption to genital epithelial HEC-1A cell line, on HIV transcytosis throughout a monolayer of polarized epithelial HEC-1A cells, on HIV adsorption to MDDC and on transfer of HIV from MDDC to autologous T lymphocytes. RESULTS: HHA faintly inhibited attachment to HEC-1A cells of the R5-tropic HIV-1(Ba-L )strain, in a dose-dependent manner, whereas GNA moderately inhibited HIV adsorption in the same context, but only at high drug doses. Only HHA, but not GNA, inhibited HIV-1(JR-CSF )transcytosis in a dose-dependent manner. By confocal microscopy, HHA, but not GNA, was adsorbed at the epithelial cell surface, suggesting that HHA interacts specifically with receptors mediating HIV-1 transcytosis. Both plant lectins partially inhibited HIV attachment to MDDC. HHA inhibited more efficiently the transfer of HIV from MDDC to T cell, than GNA. Both HHA and GNA lacked toxicity below 200 μg/ml irrespective the cellular system used and do not disturb the monolayer integrity of epithelial cells. CONCLUSION: These observations demonstrate higher inhibitory activities of the lectin plant HHA by comparison to GNA, on HIV adsorption to HEC-1A cell line, HIV transcytosis through HEC-1A cell line monolayer, HIV adsorption to MDDC and HIV transfer from MDDC to T cells, highlighting the potential interest of HHA as effective microbicide against HIV.
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spelling pubmed-19041812007-06-29 Differential in vitro inhibitory activity against HIV-1 of alpha-(1-3)- and alpha-(1-6)-D-mannose specific plant lectins : Implication for microbicide development Saïdi, Hela Nasreddine, Nadine Jenabian, Mohammad-Ali Lecerf, Maxime Schols, Dominique Krief, Corinne Balzarini, Jan Bélec, Laurent J Transl Med Research BACKGROUND: Plant lectins such as Galanthus nivalis agglutinin (GNA) and Hippeastrum hybrid agglutinin (HHA) are natural proteins able to link mannose residues, and therefore inhibit HIV-target cell interactions. Plant lectins are candidate for microbicide development. OBJECTIVE: To evaluate the activity against HIV of the mannose-specific plant lectins HHA and GNA at the cellular membrane level of epithelial cells and monocyte-derived dendritic cells (MDDC), two potential target cells of HIV at the genital mucosal level. METHODS: The inhibitory effects of HHA and GNA were evaluated on HIV adsorption to genital epithelial HEC-1A cell line, on HIV transcytosis throughout a monolayer of polarized epithelial HEC-1A cells, on HIV adsorption to MDDC and on transfer of HIV from MDDC to autologous T lymphocytes. RESULTS: HHA faintly inhibited attachment to HEC-1A cells of the R5-tropic HIV-1(Ba-L )strain, in a dose-dependent manner, whereas GNA moderately inhibited HIV adsorption in the same context, but only at high drug doses. Only HHA, but not GNA, inhibited HIV-1(JR-CSF )transcytosis in a dose-dependent manner. By confocal microscopy, HHA, but not GNA, was adsorbed at the epithelial cell surface, suggesting that HHA interacts specifically with receptors mediating HIV-1 transcytosis. Both plant lectins partially inhibited HIV attachment to MDDC. HHA inhibited more efficiently the transfer of HIV from MDDC to T cell, than GNA. Both HHA and GNA lacked toxicity below 200 μg/ml irrespective the cellular system used and do not disturb the monolayer integrity of epithelial cells. CONCLUSION: These observations demonstrate higher inhibitory activities of the lectin plant HHA by comparison to GNA, on HIV adsorption to HEC-1A cell line, HIV transcytosis through HEC-1A cell line monolayer, HIV adsorption to MDDC and HIV transfer from MDDC to T cells, highlighting the potential interest of HHA as effective microbicide against HIV. BioMed Central 2007-06-12 /pmc/articles/PMC1904181/ /pubmed/17565674 http://dx.doi.org/10.1186/1479-5876-5-28 Text en Copyright © 2007 Saïdi et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Saïdi, Hela
Nasreddine, Nadine
Jenabian, Mohammad-Ali
Lecerf, Maxime
Schols, Dominique
Krief, Corinne
Balzarini, Jan
Bélec, Laurent
Differential in vitro inhibitory activity against HIV-1 of alpha-(1-3)- and alpha-(1-6)-D-mannose specific plant lectins : Implication for microbicide development
title Differential in vitro inhibitory activity against HIV-1 of alpha-(1-3)- and alpha-(1-6)-D-mannose specific plant lectins : Implication for microbicide development
title_full Differential in vitro inhibitory activity against HIV-1 of alpha-(1-3)- and alpha-(1-6)-D-mannose specific plant lectins : Implication for microbicide development
title_fullStr Differential in vitro inhibitory activity against HIV-1 of alpha-(1-3)- and alpha-(1-6)-D-mannose specific plant lectins : Implication for microbicide development
title_full_unstemmed Differential in vitro inhibitory activity against HIV-1 of alpha-(1-3)- and alpha-(1-6)-D-mannose specific plant lectins : Implication for microbicide development
title_short Differential in vitro inhibitory activity against HIV-1 of alpha-(1-3)- and alpha-(1-6)-D-mannose specific plant lectins : Implication for microbicide development
title_sort differential in vitro inhibitory activity against hiv-1 of alpha-(1-3)- and alpha-(1-6)-d-mannose specific plant lectins : implication for microbicide development
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1904181/
https://www.ncbi.nlm.nih.gov/pubmed/17565674
http://dx.doi.org/10.1186/1479-5876-5-28
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