Isolation and characterization of mutated alcohol oxidases from the yeast Hansenula polymorpha with decreased affinity toward substrates and their use as selective elements of an amperometric biosensor

BACKGROUND: Accurate, rapid, and economic on-line analysis of ethanol is very desirable. However, available biosensors achieve saturation at very low ethanol concentrations and thus demand the time and labour consuming procedure of sample dilution. RESULTS: Hansenula polymorpha (Pichia angusta) muta...

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Detalles Bibliográficos
Autores principales: Dmytruk, Kostyantyn V, Smutok, Oleh V, Ryabova, Olena B, Gayda, Galyna Z, Sibirny, Volodymyr A, Schuhmann, Wolfgang, Gonchar, Mykhailo V, Sibirny, Andriy A
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2007
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1905912/
https://www.ncbi.nlm.nih.gov/pubmed/17567895
http://dx.doi.org/10.1186/1472-6750-7-33

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