Cargando…

CD(8+ )T lymphocytes in bronchoalveolar lavage in idiopathic pulmonary fibrosis

BACKGROUND: Recently it was shown that in Idiopathic Pulmonary Fibrosis (IPF) tissue infiltrating CD(8+ )T lymphocytes (TLs) are associated with breathlessness and physiological indices of disease severity, as well as that CD(8+ )TLs recovered by bronchoalveolar lavage (BAL) relate to those infiltra...

Descripción completa

Detalles Bibliográficos
Autores principales: Papiris, Spyros A, Kollintza, Androniki, Karatza, Marilena, Manali, Effrosyni D, Sotiropoulou, Christina, Milic-Emili, Joseph, Roussos, Charis, Daniil, Zoe
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1906752/
https://www.ncbi.nlm.nih.gov/pubmed/17578573
http://dx.doi.org/10.1186/1476-9255-4-14
_version_ 1782134018902327296
author Papiris, Spyros A
Kollintza, Androniki
Karatza, Marilena
Manali, Effrosyni D
Sotiropoulou, Christina
Milic-Emili, Joseph
Roussos, Charis
Daniil, Zoe
author_facet Papiris, Spyros A
Kollintza, Androniki
Karatza, Marilena
Manali, Effrosyni D
Sotiropoulou, Christina
Milic-Emili, Joseph
Roussos, Charis
Daniil, Zoe
author_sort Papiris, Spyros A
collection PubMed
description BACKGROUND: Recently it was shown that in Idiopathic Pulmonary Fibrosis (IPF) tissue infiltrating CD(8+ )T lymphocytes (TLs) are associated with breathlessness and physiological indices of disease severity, as well as that CD(8+ )TLs recovered by bronchoalveolar lavage (BAL) relate to those infiltrating lung tissue. Since BAL is a far less invasive technique than tissue biopsy to study mechanisms in IPF we further investigated the usefulness offered by this means by studying the relationship between BAL macrophages, neutrophils, eosinophils, CD(3+), CD(4+), CD(8+), CD(8+/38+ )TLs and CD(4+)/CD(8+ )ratio with breathlessness and physiological indices. PATIENTS AND METHODS: 27 IPF patients, 63 ± 9 years of age were examined. Cell counts were expressed as percentages of total cells and TLs were evaluated by flow cytometry. FEV(1), FVC, TLC, RV, DLCO, PaO(2), and PaCO(2 )were measured in all. Breathlessness was assessed by the Medical Research Council (MRC) chronic dyspnoea scale. RESULTS: CD(8+ )TLs correlated positively (r(s )= 0.46, p = 0.02), while CD(4+)/CD(8+ )ratio negatively (r(s )= -0.54, p = 0.006) with the MRC grade. CD(8+ )TLs correlated negatively with RV (r(s )= -0.50, p = 0.017). CD(8+/38+ )TLs were negatively related to the FEV(1 )and FVC (r(s )= -0.53, p = 0.03 and r(s )= -0.59, p = 0.02, respectively). Neutrophils correlated positively with the MRC grade (r(s )= 0.42, p = 0.03), and negatively with the DLCO (r(s )= -0.54, p = 0.005), PaO(2 )(r(s )= -0.44, p = 0.03), and PaCO(2 )(r(s )= -0.52, p = 0.01). CONCLUSION: BAL CD(8+ )TLs associations with physiological and clinical indices seem to indicate their implication in IPF pathogenesis, confirming our previous tissue study.
format Text
id pubmed-1906752
institution National Center for Biotechnology Information
language English
publishDate 2007
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-19067522007-07-04 CD(8+ )T lymphocytes in bronchoalveolar lavage in idiopathic pulmonary fibrosis Papiris, Spyros A Kollintza, Androniki Karatza, Marilena Manali, Effrosyni D Sotiropoulou, Christina Milic-Emili, Joseph Roussos, Charis Daniil, Zoe J Inflamm (Lond) Review BACKGROUND: Recently it was shown that in Idiopathic Pulmonary Fibrosis (IPF) tissue infiltrating CD(8+ )T lymphocytes (TLs) are associated with breathlessness and physiological indices of disease severity, as well as that CD(8+ )TLs recovered by bronchoalveolar lavage (BAL) relate to those infiltrating lung tissue. Since BAL is a far less invasive technique than tissue biopsy to study mechanisms in IPF we further investigated the usefulness offered by this means by studying the relationship between BAL macrophages, neutrophils, eosinophils, CD(3+), CD(4+), CD(8+), CD(8+/38+ )TLs and CD(4+)/CD(8+ )ratio with breathlessness and physiological indices. PATIENTS AND METHODS: 27 IPF patients, 63 ± 9 years of age were examined. Cell counts were expressed as percentages of total cells and TLs were evaluated by flow cytometry. FEV(1), FVC, TLC, RV, DLCO, PaO(2), and PaCO(2 )were measured in all. Breathlessness was assessed by the Medical Research Council (MRC) chronic dyspnoea scale. RESULTS: CD(8+ )TLs correlated positively (r(s )= 0.46, p = 0.02), while CD(4+)/CD(8+ )ratio negatively (r(s )= -0.54, p = 0.006) with the MRC grade. CD(8+ )TLs correlated negatively with RV (r(s )= -0.50, p = 0.017). CD(8+/38+ )TLs were negatively related to the FEV(1 )and FVC (r(s )= -0.53, p = 0.03 and r(s )= -0.59, p = 0.02, respectively). Neutrophils correlated positively with the MRC grade (r(s )= 0.42, p = 0.03), and negatively with the DLCO (r(s )= -0.54, p = 0.005), PaO(2 )(r(s )= -0.44, p = 0.03), and PaCO(2 )(r(s )= -0.52, p = 0.01). CONCLUSION: BAL CD(8+ )TLs associations with physiological and clinical indices seem to indicate their implication in IPF pathogenesis, confirming our previous tissue study. BioMed Central 2007-06-19 /pmc/articles/PMC1906752/ /pubmed/17578573 http://dx.doi.org/10.1186/1476-9255-4-14 Text en Copyright © 2007 Papiris et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Review
Papiris, Spyros A
Kollintza, Androniki
Karatza, Marilena
Manali, Effrosyni D
Sotiropoulou, Christina
Milic-Emili, Joseph
Roussos, Charis
Daniil, Zoe
CD(8+ )T lymphocytes in bronchoalveolar lavage in idiopathic pulmonary fibrosis
title CD(8+ )T lymphocytes in bronchoalveolar lavage in idiopathic pulmonary fibrosis
title_full CD(8+ )T lymphocytes in bronchoalveolar lavage in idiopathic pulmonary fibrosis
title_fullStr CD(8+ )T lymphocytes in bronchoalveolar lavage in idiopathic pulmonary fibrosis
title_full_unstemmed CD(8+ )T lymphocytes in bronchoalveolar lavage in idiopathic pulmonary fibrosis
title_short CD(8+ )T lymphocytes in bronchoalveolar lavage in idiopathic pulmonary fibrosis
title_sort cd(8+ )t lymphocytes in bronchoalveolar lavage in idiopathic pulmonary fibrosis
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1906752/
https://www.ncbi.nlm.nih.gov/pubmed/17578573
http://dx.doi.org/10.1186/1476-9255-4-14
work_keys_str_mv AT papirisspyrosa cd8tlymphocytesinbronchoalveolarlavageinidiopathicpulmonaryfibrosis
AT kollintzaandroniki cd8tlymphocytesinbronchoalveolarlavageinidiopathicpulmonaryfibrosis
AT karatzamarilena cd8tlymphocytesinbronchoalveolarlavageinidiopathicpulmonaryfibrosis
AT manalieffrosynid cd8tlymphocytesinbronchoalveolarlavageinidiopathicpulmonaryfibrosis
AT sotiropoulouchristina cd8tlymphocytesinbronchoalveolarlavageinidiopathicpulmonaryfibrosis
AT milicemilijoseph cd8tlymphocytesinbronchoalveolarlavageinidiopathicpulmonaryfibrosis
AT roussoscharis cd8tlymphocytesinbronchoalveolarlavageinidiopathicpulmonaryfibrosis
AT daniilzoe cd8tlymphocytesinbronchoalveolarlavageinidiopathicpulmonaryfibrosis