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The identification and characterization of a novel protein, c19orf10, in the synovium
Joint inflammation and destruction have been linked to the deregulation of the highly synthetic fibroblast-like synoviocytes (FLSs), and much of our current understanding of the mechanisms that underlie synovitis has been collected from studies of FLSs. During a proteomic analysis of FLS cells, we i...
Autores principales: | , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2007
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1906808/ https://www.ncbi.nlm.nih.gov/pubmed/17362502 http://dx.doi.org/10.1186/ar2145 |
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author | Weiler, Tracey Du, Qiujiang Krokhin, Oleg Ens, Werner Standing, Ken El-Gabalawy, Hani Wilkins, John A |
author_facet | Weiler, Tracey Du, Qiujiang Krokhin, Oleg Ens, Werner Standing, Ken El-Gabalawy, Hani Wilkins, John A |
author_sort | Weiler, Tracey |
collection | PubMed |
description | Joint inflammation and destruction have been linked to the deregulation of the highly synthetic fibroblast-like synoviocytes (FLSs), and much of our current understanding of the mechanisms that underlie synovitis has been collected from studies of FLSs. During a proteomic analysis of FLS cells, we identified a novel protein, c19orf10 (chromosome 19 open reading frame 10), that was produced in significant amounts by these cells. The present study provides a partial characterization of c19orf10 in FLSs, synovial fluid, and the synovium. Murine monoclonal and chicken polyclonal antibodies were produced against recombinant human c19orf10 protein and used to examine the distribution of c19orf10 in cultured FLSs and in synovial tissue sections from patients with rheumatoid arthritis or osteoarthritis. The intracellular staining pattern of c19orf10 is consistent with localization in the endoplasmic reticulum/Golgi distribution. Sections of rheumatoid arthritis and osteoarthritis synovia expressed similar patterns of c19orf10 distribution with perivascular and synovial lining staining. Double-staining in situ analysis suggests that fibroblast-like synovial cells produced c19orf10, whereas macrophages, B cells, or T cells produced little or none of this protein. There is evidence of secretion into the vascular space and the extracellular matrix surrounding the synovial lining. A competitive enzyme-linked immunosorbent assay confirmed the presence of microgram levels of c19orf10 in the synovial fluids of patients with one of various arthropathies. Collectively, these results suggest that c19orf10 is an FLS-derived protein that is secreted into the synovial fluid. However, the significance of this protein in synovial biology remains to be determined. The absence of known structural motifs or domains and its relatively late evolutionary appearance raise interesting questions about its function. |
format | Text |
id | pubmed-1906808 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2007 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-19068082007-07-04 The identification and characterization of a novel protein, c19orf10, in the synovium Weiler, Tracey Du, Qiujiang Krokhin, Oleg Ens, Werner Standing, Ken El-Gabalawy, Hani Wilkins, John A Arthritis Res Ther Research Article Joint inflammation and destruction have been linked to the deregulation of the highly synthetic fibroblast-like synoviocytes (FLSs), and much of our current understanding of the mechanisms that underlie synovitis has been collected from studies of FLSs. During a proteomic analysis of FLS cells, we identified a novel protein, c19orf10 (chromosome 19 open reading frame 10), that was produced in significant amounts by these cells. The present study provides a partial characterization of c19orf10 in FLSs, synovial fluid, and the synovium. Murine monoclonal and chicken polyclonal antibodies were produced against recombinant human c19orf10 protein and used to examine the distribution of c19orf10 in cultured FLSs and in synovial tissue sections from patients with rheumatoid arthritis or osteoarthritis. The intracellular staining pattern of c19orf10 is consistent with localization in the endoplasmic reticulum/Golgi distribution. Sections of rheumatoid arthritis and osteoarthritis synovia expressed similar patterns of c19orf10 distribution with perivascular and synovial lining staining. Double-staining in situ analysis suggests that fibroblast-like synovial cells produced c19orf10, whereas macrophages, B cells, or T cells produced little or none of this protein. There is evidence of secretion into the vascular space and the extracellular matrix surrounding the synovial lining. A competitive enzyme-linked immunosorbent assay confirmed the presence of microgram levels of c19orf10 in the synovial fluids of patients with one of various arthropathies. Collectively, these results suggest that c19orf10 is an FLS-derived protein that is secreted into the synovial fluid. However, the significance of this protein in synovial biology remains to be determined. The absence of known structural motifs or domains and its relatively late evolutionary appearance raise interesting questions about its function. BioMed Central 2007 2007-03-15 /pmc/articles/PMC1906808/ /pubmed/17362502 http://dx.doi.org/10.1186/ar2145 Text en Copyright © 2007 Weiler et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Weiler, Tracey Du, Qiujiang Krokhin, Oleg Ens, Werner Standing, Ken El-Gabalawy, Hani Wilkins, John A The identification and characterization of a novel protein, c19orf10, in the synovium |
title | The identification and characterization of a novel protein, c19orf10, in the synovium |
title_full | The identification and characterization of a novel protein, c19orf10, in the synovium |
title_fullStr | The identification and characterization of a novel protein, c19orf10, in the synovium |
title_full_unstemmed | The identification and characterization of a novel protein, c19orf10, in the synovium |
title_short | The identification and characterization of a novel protein, c19orf10, in the synovium |
title_sort | identification and characterization of a novel protein, c19orf10, in the synovium |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1906808/ https://www.ncbi.nlm.nih.gov/pubmed/17362502 http://dx.doi.org/10.1186/ar2145 |
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