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The adapter importin-α provides flexible control of nuclear import at the expense of efficiency

Although there exists a large family of nuclear transport receptors (Karyopherins), the majority of known import cargoes use an adapter protein, Importin-α (Impα), which links the cargo to a karyopherin, Importin-β (Impβ). The reason for the existence of transport adapters is unknown. One hypothesis...

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Detalles Bibliográficos
Autores principales: Riddick, Greg, Macara, Ian G
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1911202/
https://www.ncbi.nlm.nih.gov/pubmed/17551513
http://dx.doi.org/10.1038/msb4100160
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author Riddick, Greg
Macara, Ian G
author_facet Riddick, Greg
Macara, Ian G
author_sort Riddick, Greg
collection PubMed
description Although there exists a large family of nuclear transport receptors (Karyopherins), the majority of known import cargoes use an adapter protein, Importin-α (Impα), which links the cargo to a karyopherin, Importin-β (Impβ). The reason for the existence of transport adapters is unknown. One hypothesis is that, as Impα re-export is coupled to GTP hydrolysis, it can drive a higher concentration of nuclear cargo than could be achieved by direct cargo binding to Importin-β. However, computer simulations predicted the opposite outcome, and showed that direct transport is faster than adapter-mediated transport. These predictions were validated experimentally. The data, together with previous analyses of nuclear protein import, suggest that the use of adapters such as importin-α provides the cell with increased dynamic range for control of nuclear import rates, but at the expense of efficiency.
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spelling pubmed-19112022007-07-09 The adapter importin-α provides flexible control of nuclear import at the expense of efficiency Riddick, Greg Macara, Ian G Mol Syst Biol Report Although there exists a large family of nuclear transport receptors (Karyopherins), the majority of known import cargoes use an adapter protein, Importin-α (Impα), which links the cargo to a karyopherin, Importin-β (Impβ). The reason for the existence of transport adapters is unknown. One hypothesis is that, as Impα re-export is coupled to GTP hydrolysis, it can drive a higher concentration of nuclear cargo than could be achieved by direct cargo binding to Importin-β. However, computer simulations predicted the opposite outcome, and showed that direct transport is faster than adapter-mediated transport. These predictions were validated experimentally. The data, together with previous analyses of nuclear protein import, suggest that the use of adapters such as importin-α provides the cell with increased dynamic range for control of nuclear import rates, but at the expense of efficiency. Nature Publishing Group 2007-06-05 /pmc/articles/PMC1911202/ /pubmed/17551513 http://dx.doi.org/10.1038/msb4100160 Text en Copyright © 2007, EMBO and Nature Publishing Group http://creativecommons.org/licenses/by-nc-nd/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits distribution, and reproduction in any medium, provided the original author and source are credited. This license does not permit commercial exploitation or the creation of derivative works without specific permission.
spellingShingle Report
Riddick, Greg
Macara, Ian G
The adapter importin-α provides flexible control of nuclear import at the expense of efficiency
title The adapter importin-α provides flexible control of nuclear import at the expense of efficiency
title_full The adapter importin-α provides flexible control of nuclear import at the expense of efficiency
title_fullStr The adapter importin-α provides flexible control of nuclear import at the expense of efficiency
title_full_unstemmed The adapter importin-α provides flexible control of nuclear import at the expense of efficiency
title_short The adapter importin-α provides flexible control of nuclear import at the expense of efficiency
title_sort adapter importin-α provides flexible control of nuclear import at the expense of efficiency
topic Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1911202/
https://www.ncbi.nlm.nih.gov/pubmed/17551513
http://dx.doi.org/10.1038/msb4100160
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