Cargando…
The adapter importin-α provides flexible control of nuclear import at the expense of efficiency
Although there exists a large family of nuclear transport receptors (Karyopherins), the majority of known import cargoes use an adapter protein, Importin-α (Impα), which links the cargo to a karyopherin, Importin-β (Impβ). The reason for the existence of transport adapters is unknown. One hypothesis...
Autores principales: | , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2007
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1911202/ https://www.ncbi.nlm.nih.gov/pubmed/17551513 http://dx.doi.org/10.1038/msb4100160 |
_version_ | 1782134052774477824 |
---|---|
author | Riddick, Greg Macara, Ian G |
author_facet | Riddick, Greg Macara, Ian G |
author_sort | Riddick, Greg |
collection | PubMed |
description | Although there exists a large family of nuclear transport receptors (Karyopherins), the majority of known import cargoes use an adapter protein, Importin-α (Impα), which links the cargo to a karyopherin, Importin-β (Impβ). The reason for the existence of transport adapters is unknown. One hypothesis is that, as Impα re-export is coupled to GTP hydrolysis, it can drive a higher concentration of nuclear cargo than could be achieved by direct cargo binding to Importin-β. However, computer simulations predicted the opposite outcome, and showed that direct transport is faster than adapter-mediated transport. These predictions were validated experimentally. The data, together with previous analyses of nuclear protein import, suggest that the use of adapters such as importin-α provides the cell with increased dynamic range for control of nuclear import rates, but at the expense of efficiency. |
format | Text |
id | pubmed-1911202 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2007 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-19112022007-07-09 The adapter importin-α provides flexible control of nuclear import at the expense of efficiency Riddick, Greg Macara, Ian G Mol Syst Biol Report Although there exists a large family of nuclear transport receptors (Karyopherins), the majority of known import cargoes use an adapter protein, Importin-α (Impα), which links the cargo to a karyopherin, Importin-β (Impβ). The reason for the existence of transport adapters is unknown. One hypothesis is that, as Impα re-export is coupled to GTP hydrolysis, it can drive a higher concentration of nuclear cargo than could be achieved by direct cargo binding to Importin-β. However, computer simulations predicted the opposite outcome, and showed that direct transport is faster than adapter-mediated transport. These predictions were validated experimentally. The data, together with previous analyses of nuclear protein import, suggest that the use of adapters such as importin-α provides the cell with increased dynamic range for control of nuclear import rates, but at the expense of efficiency. Nature Publishing Group 2007-06-05 /pmc/articles/PMC1911202/ /pubmed/17551513 http://dx.doi.org/10.1038/msb4100160 Text en Copyright © 2007, EMBO and Nature Publishing Group http://creativecommons.org/licenses/by-nc-nd/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits distribution, and reproduction in any medium, provided the original author and source are credited. This license does not permit commercial exploitation or the creation of derivative works without specific permission. |
spellingShingle | Report Riddick, Greg Macara, Ian G The adapter importin-α provides flexible control of nuclear import at the expense of efficiency |
title | The adapter importin-α provides flexible control of nuclear import at the expense of efficiency |
title_full | The adapter importin-α provides flexible control of nuclear import at the expense of efficiency |
title_fullStr | The adapter importin-α provides flexible control of nuclear import at the expense of efficiency |
title_full_unstemmed | The adapter importin-α provides flexible control of nuclear import at the expense of efficiency |
title_short | The adapter importin-α provides flexible control of nuclear import at the expense of efficiency |
title_sort | adapter importin-α provides flexible control of nuclear import at the expense of efficiency |
topic | Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1911202/ https://www.ncbi.nlm.nih.gov/pubmed/17551513 http://dx.doi.org/10.1038/msb4100160 |
work_keys_str_mv | AT riddickgreg theadapterimportinaprovidesflexiblecontrolofnuclearimportattheexpenseofefficiency AT macaraiang theadapterimportinaprovidesflexiblecontrolofnuclearimportattheexpenseofefficiency AT riddickgreg adapterimportinaprovidesflexiblecontrolofnuclearimportattheexpenseofefficiency AT macaraiang adapterimportinaprovidesflexiblecontrolofnuclearimportattheexpenseofefficiency |