Sensitive Spectroscopic Detection of Large and Denatured Protein Aggregates in Solution by Use of the Fluorescent Dye Nile Red

The fluorescent dye Nile red was used as a probe for the sensitive detection of large, denatured aggregates of the model protein β-galactosidase (E. coli) in solution. Aggregates were formed by irreversible heat denaturation of β-galactosidase below and above the protein’s unfolding temperature of 5...

Descripción completa

Detalles Bibliográficos
Autores principales: Sutter, Marc, Oliveira, Sabrina, Sanders, Niek N., Lucas, Bart, van Hoek, Arie, Hink, Mark A., Visser, Antonie J. W. G., De Smedt, Stefaan C., Hennink, Wim E., Jiskoot, Wim
Formato: Texto
Lenguaje:English
Publicado: Kluwer Academic Publishers-Plenum Publishers 2007
Materias:
Original Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1915606/
https://www.ncbi.nlm.nih.gov/pubmed/17294134
http://dx.doi.org/10.1007/s10895-007-0156-6

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1915606/
https://www.ncbi.nlm.nih.gov/pubmed/17294134
http://dx.doi.org/10.1007/s10895-007-0156-6

Ejemplares similares