Novel Peptide Sequence (“IQ-tag”) with High Affinity for NIR Fluorochromes Allows Protein and Cell Specific Labeling for In Vivo Imaging

BACKGROUND: Probes that allow site-specific protein labeling have become critical tools for visualizing biological processes. METHODS: Here we used phage display to identify a novel peptide sequence with nanomolar affinity for near infrared (NIR) (benz)indolium fluorochromes. The developed peptide s...

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Detalles Bibliográficos
Autores principales: Kelly, Kimberly A., Carson, Jonathan, McCarthy, Jason R., Weissleder, Ralph
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2007
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1919420/
https://www.ncbi.nlm.nih.gov/pubmed/17653285
http://dx.doi.org/10.1371/journal.pone.0000665
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author Kelly, Kimberly A.
Carson, Jonathan
McCarthy, Jason R.
Weissleder, Ralph
author_facet Kelly, Kimberly A.
Carson, Jonathan
McCarthy, Jason R.
Weissleder, Ralph
author_sort Kelly, Kimberly A.
collection PubMed
description BACKGROUND: Probes that allow site-specific protein labeling have become critical tools for visualizing biological processes. METHODS: Here we used phage display to identify a novel peptide sequence with nanomolar affinity for near infrared (NIR) (benz)indolium fluorochromes. The developed peptide sequence (“IQ-tag”) allows detection of NIR dyes in a wide range of assays including ELISA, flow cytometry, high throughput screens, microscopy, and optical in vivo imaging. SIGNIFICANCE: The described method is expected to have broad utility in numerous applications, namely site-specific protein imaging, target identification, cell tracking, and drug development.
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spelling pubmed-19194202007-07-26 Novel Peptide Sequence (“IQ-tag”) with High Affinity for NIR Fluorochromes Allows Protein and Cell Specific Labeling for In Vivo Imaging Kelly, Kimberly A. Carson, Jonathan McCarthy, Jason R. Weissleder, Ralph PLoS One Research Article BACKGROUND: Probes that allow site-specific protein labeling have become critical tools for visualizing biological processes. METHODS: Here we used phage display to identify a novel peptide sequence with nanomolar affinity for near infrared (NIR) (benz)indolium fluorochromes. The developed peptide sequence (“IQ-tag”) allows detection of NIR dyes in a wide range of assays including ELISA, flow cytometry, high throughput screens, microscopy, and optical in vivo imaging. SIGNIFICANCE: The described method is expected to have broad utility in numerous applications, namely site-specific protein imaging, target identification, cell tracking, and drug development. Public Library of Science 2007-07-25 /pmc/articles/PMC1919420/ /pubmed/17653285 http://dx.doi.org/10.1371/journal.pone.0000665 Text en Kelly et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Kelly, Kimberly A.
Carson, Jonathan
McCarthy, Jason R.
Weissleder, Ralph
Novel Peptide Sequence (“IQ-tag”) with High Affinity for NIR Fluorochromes Allows Protein and Cell Specific Labeling for In Vivo Imaging
title Novel Peptide Sequence (“IQ-tag”) with High Affinity for NIR Fluorochromes Allows Protein and Cell Specific Labeling for In Vivo Imaging
title_full Novel Peptide Sequence (“IQ-tag”) with High Affinity for NIR Fluorochromes Allows Protein and Cell Specific Labeling for In Vivo Imaging
title_fullStr Novel Peptide Sequence (“IQ-tag”) with High Affinity for NIR Fluorochromes Allows Protein and Cell Specific Labeling for In Vivo Imaging
title_full_unstemmed Novel Peptide Sequence (“IQ-tag”) with High Affinity for NIR Fluorochromes Allows Protein and Cell Specific Labeling for In Vivo Imaging
title_short Novel Peptide Sequence (“IQ-tag”) with High Affinity for NIR Fluorochromes Allows Protein and Cell Specific Labeling for In Vivo Imaging
title_sort novel peptide sequence (“iq-tag”) with high affinity for nir fluorochromes allows protein and cell specific labeling for in vivo imaging
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1919420/
https://www.ncbi.nlm.nih.gov/pubmed/17653285
http://dx.doi.org/10.1371/journal.pone.0000665
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