Cargando…

Phosphorylation in the serine/threonine 2609–2647 cluster promotes but is not essential for DNA-dependent protein kinase-mediated nonhomologous end joining in human whole-cell extracts

Previous work suggested that phosphorylation of DNA-PKcs at several serine/threonine (S/T) residues at positions 2609–2647 promotes DNA-PK-dependent end joining. In an attempt to clarify the role of such phosphorylation, end joining was examined in extracts of DNA-PKcs-deficient M059J cells. Joining...

Descripción completa

Detalles Bibliográficos
Autores principales:	Povirk, Lawrence F., Zhou, Rui-Zhe, Ramsden, Dale A., Lees-Miller, Susan P., Valerie, Kristoffer
Formato:	Texto
Lenguaje:	English
Publicado:	Oxford University Press 2007
Materias:	Nucleic Acid Enzymes
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1919499/ https://www.ncbi.nlm.nih.gov/pubmed/17526517 http://dx.doi.org/10.1093/nar/gkm339

Ejemplares similares

Requirement for XLF/Cernunnos in alignment-based gap filling by DNA polymerases λ and μ for nonhomologous end joining in human whole-cell extracts
por: Akopiants, Konstantin, et al.
Publicado: (2009)

End-bridging is required for pol μ to efficiently promote repair of noncomplementary ends by nonhomologous end joining
por: Davis, Bryan J., et al.
Publicado: (2008)

Evidence for an inositol hexakisphosphate-dependent role for Ku in mammalian nonhomologous end joining that is independent of its role in the DNA-dependent protein kinase
por: Cheung, Joyce C.Y., et al.
Publicado: (2008)

Bacterial Ligase D preternary-precatalytic complex performs efficient abasic sites processing at double strand breaks during nonhomologous end joining
por: de Ory, Ana, et al.
Publicado: (2019)

A mechanism for DNA-PK activation requiring unique contributions from each strand of a DNA terminus and implications for microhomology-mediated nonhomologous DNA end joining
por: Pawelczak, Katherine S., et al.
Publicado: (2008)

Coordinate 5′ and 3′ endonucleolytic trimming of terminally blocked blunt DNA double-strand break ends by Artemis nuclease and DNA-dependent protein kinase
por: Yannone, Steven M., et al.
Publicado: (2008)

Phosphorylation of the RNase III enzyme Drosha at Serine300 or Serine302 is required for its nuclear localization
por: Tang, Xiaoli, et al.
Publicado: (2010)

Casein kinase I δ/ɛ phosphorylates topoisomerase IIα at serine-1106 and modulates DNA cleavage activity
por: Grozav, Adrian G., et al.
Publicado: (2009)

Mismatch discrimination and sequence bias during end-joining by DNA ligases
por: Bilotti, Katharina, et al.
Publicado: (2022)

Ribonucleotides and manganese ions improve non-homologous end joining by human Polµ
por: Martin, Maria Jose, et al.
Publicado: (2013)

Tolerance for 8-oxoguanine but not thymine glycol in alignment-based gap filling of partially complementary double-strand break ends by DNA polymerase λ in human nuclear extracts
por: Zhou, Rui-Zhe, et al.
Publicado: (2008)

Human DNA ligase III bridges two DNA ends to promote specific intermolecular DNA end joining
por: Kukshal, Vandna, et al.
Publicado: (2015)

Plant organellar DNA polymerases repair double-stranded breaks by microhomology-mediated end-joining
por: García-Medel, Paola L, et al.
Publicado: (2019)

Rad51 and RecA juxtapose dsDNA ends ready for DNA ligase-catalyzed end-joining under recombinase-suppressive conditions
por: Konomura, Naoto, et al.
Publicado: (2017)

Human DNA ligases I and III have stand-alone end-joining capability, but differ in ligation efficiency and specificity
por: McNally, Justin R, et al.
Publicado: (2023)

Duplex strand joining reactions catalyzed by vaccinia virus DNA polymerase
por: Hamilton, Michael D., et al.
Publicado: (2007)

Coiled-coil interactions mediate serine integrase directionality
por: Gupta, Kushol, et al.
Publicado: (2017)

A specific loop in human DNA polymerase mu allows switching between creative and DNA-instructed synthesis
por: Juárez, Raquel, et al.
Publicado: (2006)

ATM-independent, high-fidelity nonhomologous end joining predominates in human embryonic stem cells
por: Adams, Bret R., et al.
Publicado: (2010)

Requirements for 5′dRP/AP lyase activity in Ku
por: Strande, Natasha T., et al.
Publicado: (2014)

Crossover-site sequence and DNA torsional stress control strand interchanges by the Bxb1 site-specific serine recombinase
por: Keenholtz, Ross A., et al.
Publicado: (2016)

Substrate preference of Gen endonucleases highlights the importance of branched structures as DNA damage repair intermediates
por: Bellendir, Stephanie P., et al.
Publicado: (2017)

Site specific phosphorylation of yeast RNA polymerase I
por: Gerber, Jochen, et al.
Publicado: (2008)

Nicked-site substrates for a serine recombinase reveal enzyme–DNA communications and an essential tethering role of covalent enzyme–DNA linkages
por: Olorunniji, Femi J., et al.
Publicado: (2015)

Reversal of DNA damage induced Topoisomerase 2 DNA–protein crosslinks by Tdp2
por: Schellenberg, Matthew J., et al.
Publicado: (2016)

Cockayne syndrome protein B interacts with and is phosphorylated by c-Abl tyrosine kinase
por: Imam, Syed Z., et al.
Publicado: (2007)

Acetylation and phosphorylation of human TFAM regulate TFAM–DNA interactions via contrasting mechanisms
por: King, Graeme A, et al.
Publicado: (2018)

Lewis acid catalysis of phosphoryl transfer from a copper(II)-NTP complex in a kinase ribozyme
por: Biondi, Elisa, et al.
Publicado: (2013)

VapCs of Mycobacterium tuberculosis cleave RNAs essential for translation
por: Winther, Kristoffer, et al.
Publicado: (2016)

Phosphorylation of SAMHD1 Thr592 increases C-terminal domain dynamics, tetramer dissociation and ssDNA binding kinetics
por: Orris, Benjamin, et al.
Publicado: (2022)

The C nucleotide at the mature 5′ end of the Escherichia coli proline tRNAs is required for the RNase E cleavage specificity at the 3′ terminus as well as functionality
por: Mohanty, Bijoy K, et al.
Publicado: (2022)

The interplay of primer-template DNA phosphorylation status and single-stranded DNA binding proteins in directing clamp loaders to the appropriate polarity of DNA
por: Hayner, Jaclyn N., et al.
Publicado: (2014)

cAMP protein kinase phosphorylates the Mos1 transposase and regulates its activity: evidences from mass spectrometry and biochemical analyses
por: Bouchet, Nicolas, et al.
Publicado: (2014)

TDP1 suppresses mis-joining of radiomimetic DNA double-strand breaks and cooperates with Artemis to promote optimal nonhomologous end joining
por: Kawale, Ajinkya S, et al.
Publicado: (2018)

Resolution of complex ends by Nonhomologous end joining - better to be lucky than good?
por: Strande, Natasha Tiffany, et al.
Publicado: (2012)

The DNA binding and 3′-end preferential activity of human tyrosyl-DNA phosphodiesterase
por: Dexheimer, Thomas S., et al.
Publicado: (2010)

Mutational and biochemical analysis of the DNA-entry nuclease EndA from Streptococcus pneumoniae
por: Midon, Marika, et al.
Publicado: (2011)

Role of tRNA amino acid-accepting end in aminoacylation and its quality control
por: Zhou, Xiao-Long, et al.
Publicado: (2011)

Mechanism of RNA 2′,3′-cyclic phosphate end healing by T4 polynucleotide kinase–phosphatase
por: Das, Ushati, et al.
Publicado: (2013)

Hyperactive mariner transposons are created by mutations that disrupt allosterism and increase the rate of transposon end synapsis
por: Liu, Danxu, et al.
Publicado: (2014)

Cannot write session to /tmp/vufind_sessions/sess_gsldofa39lfgqq8ud6pjahnbmv