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Identification of genes differentially expressed during larval molting and metamorphosis of Helicoverpa armigera

BACKGROUND: Larval molting and metamorphosis are important physiological processes in the life cycle of the holometabolous insect. We used suppression subtractive hybridization (SSH) to identify genes differentially expressed during larval molting and metamorphosis. RESULTS: We performed SSH between...

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Autores principales: Dong, Du-Juan, He, Hong-Juan, Chai, Lian-Qin, Jiang, Xiao-Juan, Wang, Jin-Xing, Zhao, Xiao-Fan
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1925068/
https://www.ncbi.nlm.nih.gov/pubmed/17588272
http://dx.doi.org/10.1186/1471-213X-7-73
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author Dong, Du-Juan
He, Hong-Juan
Chai, Lian-Qin
Jiang, Xiao-Juan
Wang, Jin-Xing
Zhao, Xiao-Fan
author_facet Dong, Du-Juan
He, Hong-Juan
Chai, Lian-Qin
Jiang, Xiao-Juan
Wang, Jin-Xing
Zhao, Xiao-Fan
author_sort Dong, Du-Juan
collection PubMed
description BACKGROUND: Larval molting and metamorphosis are important physiological processes in the life cycle of the holometabolous insect. We used suppression subtractive hybridization (SSH) to identify genes differentially expressed during larval molting and metamorphosis. RESULTS: We performed SSH between tissues from a variety of developmental stages, including molting 5th and feeding 6th instar larvae, metamorphically committed and feeding 5th instar larvae, and feeding 5th instar and metamorphically committed larvae. One hundred expressed sequence tags (ESTs) were identified and included 73 putative genes with similarity to known genes, and 27 unknown ESTs. SSH results were further characterized by dot blot, Northern blot, and RT-PCR. The expression levels of eleven genes were found to change during larval molting or metamorphosis, suggesting a functional role during these processes. CONCLUSION: These results provide a new set of genes expressed specifically during larval molt or metamorphosis that are candidates for further studies into the regulatory mechanisms of those stage-specific genes during larval molt and metamorphosis
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spelling pubmed-19250682007-07-20 Identification of genes differentially expressed during larval molting and metamorphosis of Helicoverpa armigera Dong, Du-Juan He, Hong-Juan Chai, Lian-Qin Jiang, Xiao-Juan Wang, Jin-Xing Zhao, Xiao-Fan BMC Dev Biol Research Article BACKGROUND: Larval molting and metamorphosis are important physiological processes in the life cycle of the holometabolous insect. We used suppression subtractive hybridization (SSH) to identify genes differentially expressed during larval molting and metamorphosis. RESULTS: We performed SSH between tissues from a variety of developmental stages, including molting 5th and feeding 6th instar larvae, metamorphically committed and feeding 5th instar larvae, and feeding 5th instar and metamorphically committed larvae. One hundred expressed sequence tags (ESTs) were identified and included 73 putative genes with similarity to known genes, and 27 unknown ESTs. SSH results were further characterized by dot blot, Northern blot, and RT-PCR. The expression levels of eleven genes were found to change during larval molting or metamorphosis, suggesting a functional role during these processes. CONCLUSION: These results provide a new set of genes expressed specifically during larval molt or metamorphosis that are candidates for further studies into the regulatory mechanisms of those stage-specific genes during larval molt and metamorphosis BioMed Central 2007-06-25 /pmc/articles/PMC1925068/ /pubmed/17588272 http://dx.doi.org/10.1186/1471-213X-7-73 Text en Copyright © 2007 Dong et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Dong, Du-Juan
He, Hong-Juan
Chai, Lian-Qin
Jiang, Xiao-Juan
Wang, Jin-Xing
Zhao, Xiao-Fan
Identification of genes differentially expressed during larval molting and metamorphosis of Helicoverpa armigera
title Identification of genes differentially expressed during larval molting and metamorphosis of Helicoverpa armigera
title_full Identification of genes differentially expressed during larval molting and metamorphosis of Helicoverpa armigera
title_fullStr Identification of genes differentially expressed during larval molting and metamorphosis of Helicoverpa armigera
title_full_unstemmed Identification of genes differentially expressed during larval molting and metamorphosis of Helicoverpa armigera
title_short Identification of genes differentially expressed during larval molting and metamorphosis of Helicoverpa armigera
title_sort identification of genes differentially expressed during larval molting and metamorphosis of helicoverpa armigera
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1925068/
https://www.ncbi.nlm.nih.gov/pubmed/17588272
http://dx.doi.org/10.1186/1471-213X-7-73
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