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Global transcriptional responses of fission and budding yeast to changes in copper and iron levels: a comparative study

BACKGROUND: Recent studies in comparative genomics demonstrate that interspecies comparison represents a powerful tool for identifying both conserved and specialized biologic processes across large evolutionary distances. All cells must adjust to environmental fluctuations in metal levels, because l...

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Autores principales: Rustici, Gabriella, van Bakel, Harm, Lackner, Daniel H, Holstege, Frank C, Wijmenga, Cisca, Bähler, Jürg, Brazma, Alvis
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1929147/
https://www.ncbi.nlm.nih.gov/pubmed/17477863
http://dx.doi.org/10.1186/gb-2007-8-5-r73
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author Rustici, Gabriella
van Bakel, Harm
Lackner, Daniel H
Holstege, Frank C
Wijmenga, Cisca
Bähler, Jürg
Brazma, Alvis
author_facet Rustici, Gabriella
van Bakel, Harm
Lackner, Daniel H
Holstege, Frank C
Wijmenga, Cisca
Bähler, Jürg
Brazma, Alvis
author_sort Rustici, Gabriella
collection PubMed
description BACKGROUND: Recent studies in comparative genomics demonstrate that interspecies comparison represents a powerful tool for identifying both conserved and specialized biologic processes across large evolutionary distances. All cells must adjust to environmental fluctuations in metal levels, because levels that are too low or too high can be detrimental. Here we explore the conservation of metal homoeostasis in two distantly related yeasts. RESULTS: We examined genome-wide gene expression responses to changing copper and iron levels in budding and fission yeast using DNA microarrays. The comparison reveals conservation of only a small core set of genes, defining the copper and iron regulons, with a larger number of additional genes being specific for each species. Novel regulatory targets were identified in Schizosaccharomyces pombe for Cuf1p (pex7 and SPAC3G6.05) and Fep1p (srx1, sib1, sib2, rds1, isu1, SPBC27B12.03c, SPAC1F8.02c, and SPBC947.05c). We also present evidence refuting a direct role of Cuf1p in the repression of genes involved in iron uptake. Remarkable differences were detected in responses of the two yeasts to excess copper, probably reflecting evolutionary adaptation to different environments. CONCLUSION: The considerable evolutionary distance between budding and fission yeast resulted in substantial diversion in the regulation of copper and iron homeostasis. Despite these differences, the conserved regulation of a core set of genes involved in the uptake of these metals provides valuable clues to key features of metal metabolism.
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spelling pubmed-19291472007-07-21 Global transcriptional responses of fission and budding yeast to changes in copper and iron levels: a comparative study Rustici, Gabriella van Bakel, Harm Lackner, Daniel H Holstege, Frank C Wijmenga, Cisca Bähler, Jürg Brazma, Alvis Genome Biol Research BACKGROUND: Recent studies in comparative genomics demonstrate that interspecies comparison represents a powerful tool for identifying both conserved and specialized biologic processes across large evolutionary distances. All cells must adjust to environmental fluctuations in metal levels, because levels that are too low or too high can be detrimental. Here we explore the conservation of metal homoeostasis in two distantly related yeasts. RESULTS: We examined genome-wide gene expression responses to changing copper and iron levels in budding and fission yeast using DNA microarrays. The comparison reveals conservation of only a small core set of genes, defining the copper and iron regulons, with a larger number of additional genes being specific for each species. Novel regulatory targets were identified in Schizosaccharomyces pombe for Cuf1p (pex7 and SPAC3G6.05) and Fep1p (srx1, sib1, sib2, rds1, isu1, SPBC27B12.03c, SPAC1F8.02c, and SPBC947.05c). We also present evidence refuting a direct role of Cuf1p in the repression of genes involved in iron uptake. Remarkable differences were detected in responses of the two yeasts to excess copper, probably reflecting evolutionary adaptation to different environments. CONCLUSION: The considerable evolutionary distance between budding and fission yeast resulted in substantial diversion in the regulation of copper and iron homeostasis. Despite these differences, the conserved regulation of a core set of genes involved in the uptake of these metals provides valuable clues to key features of metal metabolism. BioMed Central 2007 2007-05-03 /pmc/articles/PMC1929147/ /pubmed/17477863 http://dx.doi.org/10.1186/gb-2007-8-5-r73 Text en Copyright © 2007 Rustici et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Rustici, Gabriella
van Bakel, Harm
Lackner, Daniel H
Holstege, Frank C
Wijmenga, Cisca
Bähler, Jürg
Brazma, Alvis
Global transcriptional responses of fission and budding yeast to changes in copper and iron levels: a comparative study
title Global transcriptional responses of fission and budding yeast to changes in copper and iron levels: a comparative study
title_full Global transcriptional responses of fission and budding yeast to changes in copper and iron levels: a comparative study
title_fullStr Global transcriptional responses of fission and budding yeast to changes in copper and iron levels: a comparative study
title_full_unstemmed Global transcriptional responses of fission and budding yeast to changes in copper and iron levels: a comparative study
title_short Global transcriptional responses of fission and budding yeast to changes in copper and iron levels: a comparative study
title_sort global transcriptional responses of fission and budding yeast to changes in copper and iron levels: a comparative study
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1929147/
https://www.ncbi.nlm.nih.gov/pubmed/17477863
http://dx.doi.org/10.1186/gb-2007-8-5-r73
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