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Exon arrays provide accurate assessments of gene expression

We have developed a strategy for estimating gene expression on Affymetrix Exon arrays. The method includes a probe-specific background correction and a probe selection strategy in which a subset of probes with highly correlated intensities across multiple samples are chosen to summarize gene express...

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Detalles Bibliográficos
Autores principales: Kapur, Karen, Xing, Yi, Ouyang, Zhengqing, Wong, Wing Hung
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1929160/
https://www.ncbi.nlm.nih.gov/pubmed/17504534
http://dx.doi.org/10.1186/gb-2007-8-5-r82
Descripción
Sumario:We have developed a strategy for estimating gene expression on Affymetrix Exon arrays. The method includes a probe-specific background correction and a probe selection strategy in which a subset of probes with highly correlated intensities across multiple samples are chosen to summarize gene expression. Our results demonstrate that the proposed background model offers improvements over the default Affymetrix background correction and that Exon arrays may provide more accurate measurements of gene expression than traditional 3' arrays.