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OligoCalc: an online oligonucleotide properties calculator
We developed OligoCalc as a web-accessible, client-based computational engine for reporting DNA and RNA single-stranded and double-stranded properties, including molecular weight, solution concentration, melting temperature, estimated absorbance coefficients, inter-molecular self-complementarity est...
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Formato: | Texto |
Lenguaje: | English |
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Oxford University Press
2007
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1933198/ https://www.ncbi.nlm.nih.gov/pubmed/17452344 http://dx.doi.org/10.1093/nar/gkm234 |
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author | Kibbe, Warren A. |
author_facet | Kibbe, Warren A. |
author_sort | Kibbe, Warren A. |
collection | PubMed |
description | We developed OligoCalc as a web-accessible, client-based computational engine for reporting DNA and RNA single-stranded and double-stranded properties, including molecular weight, solution concentration, melting temperature, estimated absorbance coefficients, inter-molecular self-complementarity estimation and intra-molecular hairpin loop formation. OligoCalc has a familiar ‘calculator’ look and feel, making it readily understandable and usable. OligoCalc incorporates three common methods for calculating oligonucleotide-melting temperatures, including a nearest-neighbor thermodynamic model for melting temperature. Since it first came online in 1997, there have been more than 900 000 accesses of OligoCalc from nearly 200 000 distinct hosts, excluding search engines. OligoCalc is available at http://basic.northwestern.edu/biotools/OligoCalc.html, with links to the full source code, usage patterns and statistics at that link as well. |
format | Text |
id | pubmed-1933198 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2007 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-19331982007-07-31 OligoCalc: an online oligonucleotide properties calculator Kibbe, Warren A. Nucleic Acids Res Articles We developed OligoCalc as a web-accessible, client-based computational engine for reporting DNA and RNA single-stranded and double-stranded properties, including molecular weight, solution concentration, melting temperature, estimated absorbance coefficients, inter-molecular self-complementarity estimation and intra-molecular hairpin loop formation. OligoCalc has a familiar ‘calculator’ look and feel, making it readily understandable and usable. OligoCalc incorporates three common methods for calculating oligonucleotide-melting temperatures, including a nearest-neighbor thermodynamic model for melting temperature. Since it first came online in 1997, there have been more than 900 000 accesses of OligoCalc from nearly 200 000 distinct hosts, excluding search engines. OligoCalc is available at http://basic.northwestern.edu/biotools/OligoCalc.html, with links to the full source code, usage patterns and statistics at that link as well. Oxford University Press 2007-07 2007-04-22 /pmc/articles/PMC1933198/ /pubmed/17452344 http://dx.doi.org/10.1093/nar/gkm234 Text en © 2007 The Author(s) http://creativecommons.org/licenses/by-nc/2.0/uk/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Articles Kibbe, Warren A. OligoCalc: an online oligonucleotide properties calculator |
title | OligoCalc: an online oligonucleotide properties calculator |
title_full | OligoCalc: an online oligonucleotide properties calculator |
title_fullStr | OligoCalc: an online oligonucleotide properties calculator |
title_full_unstemmed | OligoCalc: an online oligonucleotide properties calculator |
title_short | OligoCalc: an online oligonucleotide properties calculator |
title_sort | oligocalc: an online oligonucleotide properties calculator |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1933198/ https://www.ncbi.nlm.nih.gov/pubmed/17452344 http://dx.doi.org/10.1093/nar/gkm234 |
work_keys_str_mv | AT kibbewarrena oligocalcanonlineoligonucleotidepropertiescalculator |