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NTMG (N-terminal Truncated Mutants Generator for cDNA): an automatic multiplex PCR assays design for generating various N-terminal truncated cDNA mutants
The sequential deletion method is generally used to locate the functional domain of a protein. With this method, in order to find the various N-terminal truncated mutants, researchers have to investigate the ATG-like codons, to design various multiplex polymerase chain reaction (PCR) forward primers...
Autores principales: | Chen, Yung-Fu, Chen, Rung-Ching, Tseng, Lin-Yu, Lin, Elong, Chan, Yung-Kuan, Pan, Ren-Hao |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2007
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1933230/ https://www.ncbi.nlm.nih.gov/pubmed/17488836 http://dx.doi.org/10.1093/nar/gkm305 |
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