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A major genetic locus controlling natural Plasmodium falciparum infection is shared by East and West African Anopheles gambiae

BACKGROUND: Genetic linkage mapping identified a region of chromosome 2L in the Anopheles gambiae genome that exerts major control over natural infection by Plasmodium falciparum. This 2L Plasmodium-resistance interval was mapped in mosquitoes from a natural population in Mali, West Africa, and cont...

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Autores principales: Riehle, Michelle M, Markianos, Kyriacos, Lambrechts, Louis, Xia, Ai, Sharakhov, Igor, Koella, Jacob C, Vernick, Kenneth D
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1936428/
https://www.ncbi.nlm.nih.gov/pubmed/17612409
http://dx.doi.org/10.1186/1475-2875-6-87
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author Riehle, Michelle M
Markianos, Kyriacos
Lambrechts, Louis
Xia, Ai
Sharakhov, Igor
Koella, Jacob C
Vernick, Kenneth D
author_facet Riehle, Michelle M
Markianos, Kyriacos
Lambrechts, Louis
Xia, Ai
Sharakhov, Igor
Koella, Jacob C
Vernick, Kenneth D
author_sort Riehle, Michelle M
collection PubMed
description BACKGROUND: Genetic linkage mapping identified a region of chromosome 2L in the Anopheles gambiae genome that exerts major control over natural infection by Plasmodium falciparum. This 2L Plasmodium-resistance interval was mapped in mosquitoes from a natural population in Mali, West Africa, and controls the numbers of P. falciparum oocysts that develop on the vector midgut. An important question is whether genetic variation with respect to Plasmodium-resistance exists across Africa, and if so whether the same or multiple geographically distinct resistance mechanisms are responsible for the trait. METHODS: To identify P falciparum resistance loci in pedigrees generated and infected in Kenya, East Africa, 28 microsatellite loci were typed across the mosquito genome. Genetic linkage mapping was used to detect significant linkage between genotype and numbers of midgut oocysts surviving to 7–8 days post-infection. RESULTS: A major malaria-control locus was identified on chromosome 2L in East African mosquitoes, in the same apparent position originally identified from the West African population. Presence of this resistance locus explains 75% of parasite free mosquitoes. The Kenyan resistance locus is named EA_Pfin1 (East Africa_ Plasmodium falciparum Infection Intensity). CONCLUSION: Detection of a malaria-control locus at the same chromosomal location in both East and West African mosquitoes indicates that, to the level of genetic resolution of the analysis, the same mechanism of Plasmodium-resistance, or a mechanism controlled by the same genomic region, is found across Africa, and thus probably operates in A. gambiae throughout its entire range.
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spelling pubmed-19364282007-08-01 A major genetic locus controlling natural Plasmodium falciparum infection is shared by East and West African Anopheles gambiae Riehle, Michelle M Markianos, Kyriacos Lambrechts, Louis Xia, Ai Sharakhov, Igor Koella, Jacob C Vernick, Kenneth D Malar J Research BACKGROUND: Genetic linkage mapping identified a region of chromosome 2L in the Anopheles gambiae genome that exerts major control over natural infection by Plasmodium falciparum. This 2L Plasmodium-resistance interval was mapped in mosquitoes from a natural population in Mali, West Africa, and controls the numbers of P. falciparum oocysts that develop on the vector midgut. An important question is whether genetic variation with respect to Plasmodium-resistance exists across Africa, and if so whether the same or multiple geographically distinct resistance mechanisms are responsible for the trait. METHODS: To identify P falciparum resistance loci in pedigrees generated and infected in Kenya, East Africa, 28 microsatellite loci were typed across the mosquito genome. Genetic linkage mapping was used to detect significant linkage between genotype and numbers of midgut oocysts surviving to 7–8 days post-infection. RESULTS: A major malaria-control locus was identified on chromosome 2L in East African mosquitoes, in the same apparent position originally identified from the West African population. Presence of this resistance locus explains 75% of parasite free mosquitoes. The Kenyan resistance locus is named EA_Pfin1 (East Africa_ Plasmodium falciparum Infection Intensity). CONCLUSION: Detection of a malaria-control locus at the same chromosomal location in both East and West African mosquitoes indicates that, to the level of genetic resolution of the analysis, the same mechanism of Plasmodium-resistance, or a mechanism controlled by the same genomic region, is found across Africa, and thus probably operates in A. gambiae throughout its entire range. BioMed Central 2007-07-06 /pmc/articles/PMC1936428/ /pubmed/17612409 http://dx.doi.org/10.1186/1475-2875-6-87 Text en Copyright © 2007 Riehle et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Riehle, Michelle M
Markianos, Kyriacos
Lambrechts, Louis
Xia, Ai
Sharakhov, Igor
Koella, Jacob C
Vernick, Kenneth D
A major genetic locus controlling natural Plasmodium falciparum infection is shared by East and West African Anopheles gambiae
title A major genetic locus controlling natural Plasmodium falciparum infection is shared by East and West African Anopheles gambiae
title_full A major genetic locus controlling natural Plasmodium falciparum infection is shared by East and West African Anopheles gambiae
title_fullStr A major genetic locus controlling natural Plasmodium falciparum infection is shared by East and West African Anopheles gambiae
title_full_unstemmed A major genetic locus controlling natural Plasmodium falciparum infection is shared by East and West African Anopheles gambiae
title_short A major genetic locus controlling natural Plasmodium falciparum infection is shared by East and West African Anopheles gambiae
title_sort major genetic locus controlling natural plasmodium falciparum infection is shared by east and west african anopheles gambiae
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1936428/
https://www.ncbi.nlm.nih.gov/pubmed/17612409
http://dx.doi.org/10.1186/1475-2875-6-87
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