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CD83 Modulates B Cell Function In Vitro: Increased IL-10 and Reduced Ig Secretion by CD83Tg B Cells

The murine transmembrane glycoprotein CD83 is an important regulator for both thymic T cell maturation and peripheral T cell responses. Recently, we reported that CD83 also has a function on B cells: Ubiquitous transgenic (Tg) expression of CD83 interfered with the immunoglobulin (Ig) response to in...

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Autores principales: Kretschmer, Birte, Lüthje, Katja, Guse, Andreas H., Ehrlich, Svenja, Koch-Nolte, Friedrich, Haag, Friedrich, Fleischer, Bernhard, Breloer, Minka
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1940313/
https://www.ncbi.nlm.nih.gov/pubmed/17710154
http://dx.doi.org/10.1371/journal.pone.0000755
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author Kretschmer, Birte
Lüthje, Katja
Guse, Andreas H.
Ehrlich, Svenja
Koch-Nolte, Friedrich
Haag, Friedrich
Fleischer, Bernhard
Breloer, Minka
author_facet Kretschmer, Birte
Lüthje, Katja
Guse, Andreas H.
Ehrlich, Svenja
Koch-Nolte, Friedrich
Haag, Friedrich
Fleischer, Bernhard
Breloer, Minka
author_sort Kretschmer, Birte
collection PubMed
description The murine transmembrane glycoprotein CD83 is an important regulator for both thymic T cell maturation and peripheral T cell responses. Recently, we reported that CD83 also has a function on B cells: Ubiquitous transgenic (Tg) expression of CD83 interfered with the immunoglobulin (Ig) response to infectious agents and to T cell dependent as well as T cell independent model antigen immunization. Here we compare the function of CD83Tg B cells that overexpress CD83 and CD83 mutant (CD83mu) B cells that display a drastically reduced CD83 expression. Correlating with CD83 expression, the basic as well as the lipopolysaccharide (LPS) induced expression of the activation markers CD86 and MHC-II are significantly increased in CD83Tg B cells and reciprocally decreased in CD83mu B cells. Wild-type B cells rapidly upregulate CD83 within three hours post BCR or TLR engagement by de novo protein synthesis. The forced premature overexpression of CD83 on the CD83Tg B cells results in reduced calcium signaling, reduced Ig secretion and a reciprocally increased IL-10 production upon in vitro activation. This altered phenotype is mediated by CD83 expressed on the B cells themselves, since it is observed in the absence of accessory cells. In line with this finding, purified CD83mu B cells displayed a reduced IL-10 production and slightly increased Ig secretion upon LPS stimulation in vitro. Taken together, our data strongly suggest that CD83 is expressed by B cells upon activation and contributes to the regulation of B cell function.
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spelling pubmed-19403132007-08-15 CD83 Modulates B Cell Function In Vitro: Increased IL-10 and Reduced Ig Secretion by CD83Tg B Cells Kretschmer, Birte Lüthje, Katja Guse, Andreas H. Ehrlich, Svenja Koch-Nolte, Friedrich Haag, Friedrich Fleischer, Bernhard Breloer, Minka PLoS One Research Article The murine transmembrane glycoprotein CD83 is an important regulator for both thymic T cell maturation and peripheral T cell responses. Recently, we reported that CD83 also has a function on B cells: Ubiquitous transgenic (Tg) expression of CD83 interfered with the immunoglobulin (Ig) response to infectious agents and to T cell dependent as well as T cell independent model antigen immunization. Here we compare the function of CD83Tg B cells that overexpress CD83 and CD83 mutant (CD83mu) B cells that display a drastically reduced CD83 expression. Correlating with CD83 expression, the basic as well as the lipopolysaccharide (LPS) induced expression of the activation markers CD86 and MHC-II are significantly increased in CD83Tg B cells and reciprocally decreased in CD83mu B cells. Wild-type B cells rapidly upregulate CD83 within three hours post BCR or TLR engagement by de novo protein synthesis. The forced premature overexpression of CD83 on the CD83Tg B cells results in reduced calcium signaling, reduced Ig secretion and a reciprocally increased IL-10 production upon in vitro activation. This altered phenotype is mediated by CD83 expressed on the B cells themselves, since it is observed in the absence of accessory cells. In line with this finding, purified CD83mu B cells displayed a reduced IL-10 production and slightly increased Ig secretion upon LPS stimulation in vitro. Taken together, our data strongly suggest that CD83 is expressed by B cells upon activation and contributes to the regulation of B cell function. Public Library of Science 2007-08-15 /pmc/articles/PMC1940313/ /pubmed/17710154 http://dx.doi.org/10.1371/journal.pone.0000755 Text en Kretschmer et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Kretschmer, Birte
Lüthje, Katja
Guse, Andreas H.
Ehrlich, Svenja
Koch-Nolte, Friedrich
Haag, Friedrich
Fleischer, Bernhard
Breloer, Minka
CD83 Modulates B Cell Function In Vitro: Increased IL-10 and Reduced Ig Secretion by CD83Tg B Cells
title CD83 Modulates B Cell Function In Vitro: Increased IL-10 and Reduced Ig Secretion by CD83Tg B Cells
title_full CD83 Modulates B Cell Function In Vitro: Increased IL-10 and Reduced Ig Secretion by CD83Tg B Cells
title_fullStr CD83 Modulates B Cell Function In Vitro: Increased IL-10 and Reduced Ig Secretion by CD83Tg B Cells
title_full_unstemmed CD83 Modulates B Cell Function In Vitro: Increased IL-10 and Reduced Ig Secretion by CD83Tg B Cells
title_short CD83 Modulates B Cell Function In Vitro: Increased IL-10 and Reduced Ig Secretion by CD83Tg B Cells
title_sort cd83 modulates b cell function in vitro: increased il-10 and reduced ig secretion by cd83tg b cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1940313/
https://www.ncbi.nlm.nih.gov/pubmed/17710154
http://dx.doi.org/10.1371/journal.pone.0000755
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