Cargando…

An Actin-Based Wave Generator Organizes Cell Motility

Although many of the regulators of actin assembly are known, we do not understand how these components act together to organize cell shape and movement. To address this question, we analyzed the spatial dynamics of a key actin regulator—the Scar/WAVE complex—which plays an important role in regulati...

Descripción completa

Detalles Bibliográficos
Autores principales: Weiner, Orion D, Marganski, William A, Wu, Lani F, Altschuler, Steven J, Kirschner, Marc W
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1945041/
https://www.ncbi.nlm.nih.gov/pubmed/17696648
http://dx.doi.org/10.1371/journal.pbio.0050221
_version_ 1782134485560590336
author Weiner, Orion D
Marganski, William A
Wu, Lani F
Altschuler, Steven J
Kirschner, Marc W
author_facet Weiner, Orion D
Marganski, William A
Wu, Lani F
Altschuler, Steven J
Kirschner, Marc W
author_sort Weiner, Orion D
collection PubMed
description Although many of the regulators of actin assembly are known, we do not understand how these components act together to organize cell shape and movement. To address this question, we analyzed the spatial dynamics of a key actin regulator—the Scar/WAVE complex—which plays an important role in regulating cell shape in both metazoans and plants. We have recently discovered that the Hem-1/Nap1 component of the Scar/WAVE complex localizes to propagating waves that appear to organize the leading edge of a motile immune cell, the human neutrophil. Actin is both an output and input to the Scar/WAVE complex: the complex stimulates actin assembly, and actin polymer is also required to remove the complex from the membrane. These reciprocal interactions appear to generate propagated waves of actin nucleation that exhibit many of the properties of morphogenesis in motile cells, such as the ability of cells to flow around barriers and the intricate spatial organization of protrusion at the leading edge. We propose that cell motility results from the collective behavior of multiple self-organizing waves.
format Text
id pubmed-1945041
institution National Center for Biotechnology Information
language English
publishDate 2007
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-19450412007-08-14 An Actin-Based Wave Generator Organizes Cell Motility Weiner, Orion D Marganski, William A Wu, Lani F Altschuler, Steven J Kirschner, Marc W PLoS Biol Research Article Although many of the regulators of actin assembly are known, we do not understand how these components act together to organize cell shape and movement. To address this question, we analyzed the spatial dynamics of a key actin regulator—the Scar/WAVE complex—which plays an important role in regulating cell shape in both metazoans and plants. We have recently discovered that the Hem-1/Nap1 component of the Scar/WAVE complex localizes to propagating waves that appear to organize the leading edge of a motile immune cell, the human neutrophil. Actin is both an output and input to the Scar/WAVE complex: the complex stimulates actin assembly, and actin polymer is also required to remove the complex from the membrane. These reciprocal interactions appear to generate propagated waves of actin nucleation that exhibit many of the properties of morphogenesis in motile cells, such as the ability of cells to flow around barriers and the intricate spatial organization of protrusion at the leading edge. We propose that cell motility results from the collective behavior of multiple self-organizing waves. Public Library of Science 2007-09 2007-08-14 /pmc/articles/PMC1945041/ /pubmed/17696648 http://dx.doi.org/10.1371/journal.pbio.0050221 Text en © 2007 Weiner et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Weiner, Orion D
Marganski, William A
Wu, Lani F
Altschuler, Steven J
Kirschner, Marc W
An Actin-Based Wave Generator Organizes Cell Motility
title An Actin-Based Wave Generator Organizes Cell Motility
title_full An Actin-Based Wave Generator Organizes Cell Motility
title_fullStr An Actin-Based Wave Generator Organizes Cell Motility
title_full_unstemmed An Actin-Based Wave Generator Organizes Cell Motility
title_short An Actin-Based Wave Generator Organizes Cell Motility
title_sort actin-based wave generator organizes cell motility
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1945041/
https://www.ncbi.nlm.nih.gov/pubmed/17696648
http://dx.doi.org/10.1371/journal.pbio.0050221
work_keys_str_mv AT weineroriond anactinbasedwavegeneratororganizescellmotility
AT marganskiwilliama anactinbasedwavegeneratororganizescellmotility
AT wulanif anactinbasedwavegeneratororganizescellmotility
AT altschulerstevenj anactinbasedwavegeneratororganizescellmotility
AT kirschnermarcw anactinbasedwavegeneratororganizescellmotility
AT weineroriond actinbasedwavegeneratororganizescellmotility
AT marganskiwilliama actinbasedwavegeneratororganizescellmotility
AT wulanif actinbasedwavegeneratororganizescellmotility
AT altschulerstevenj actinbasedwavegeneratororganizescellmotility
AT kirschnermarcw actinbasedwavegeneratororganizescellmotility