Cargando…

Natural Variation in the Thermotolerance of Neural Function and Behavior due to a cGMP-Dependent Protein Kinase

Although it is acknowledged that genetic variation contributes to individual differences in thermotolerance, the specific genes and pathways involved and how they are modulated by the environment remain poorly understood. We link natural variation in the thermotolerance of neural function and behavi...

Descripción completa

Detalles Bibliográficos
Autores principales: Dawson-Scully, Ken, Armstrong, Gary A.B., Kent, Clement, Robertson, R. Meldrum, Sokolowski, Marla B.
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1945089/
https://www.ncbi.nlm.nih.gov/pubmed/17712421
http://dx.doi.org/10.1371/journal.pone.0000773
_version_ 1782134488556371968
author Dawson-Scully, Ken
Armstrong, Gary A.B.
Kent, Clement
Robertson, R. Meldrum
Sokolowski, Marla B.
author_facet Dawson-Scully, Ken
Armstrong, Gary A.B.
Kent, Clement
Robertson, R. Meldrum
Sokolowski, Marla B.
author_sort Dawson-Scully, Ken
collection PubMed
description Although it is acknowledged that genetic variation contributes to individual differences in thermotolerance, the specific genes and pathways involved and how they are modulated by the environment remain poorly understood. We link natural variation in the thermotolerance of neural function and behavior in Drosophila melanogaster to the foraging gene (for, which encodes a cGMP-dependent protein kinase (PKG)) as well as to its downstream target, protein phosphatase 2A (PP2A). Genetic and pharmacological manipulations revealed that reduced PKG (or PP2A) activity caused increased thermotolerance of synaptic transmission at the larval neuromuscular junction. Like synaptic transmission, feeding movements were preserved at higher temperatures in larvae with lower PKG levels. In a comparative assay, pharmacological manipulations altering thermotolerance in a central circuit of Locusta migratoria demonstrated conservation of this neuroprotective pathway. In this circuit, either the inhibition of PKG or PP2A induced robust thermotolerance of neural function. We suggest that PKG and therefore the polymorphism associated with the allelic variation in for may provide populations with natural variation in heat stress tolerance. for's function in behavior is conserved across most organisms, including ants, bees, nematodes, and mammals. PKG's role in thermotolerance may also apply to these and other species. Natural variation in thermotolerance arising from genes involved in the PKG pathway could impact the evolution of thermotolerance in natural populations.
format Text
id pubmed-1945089
institution National Center for Biotechnology Information
language English
publishDate 2007
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-19450892007-08-22 Natural Variation in the Thermotolerance of Neural Function and Behavior due to a cGMP-Dependent Protein Kinase Dawson-Scully, Ken Armstrong, Gary A.B. Kent, Clement Robertson, R. Meldrum Sokolowski, Marla B. PLoS One Research Article Although it is acknowledged that genetic variation contributes to individual differences in thermotolerance, the specific genes and pathways involved and how they are modulated by the environment remain poorly understood. We link natural variation in the thermotolerance of neural function and behavior in Drosophila melanogaster to the foraging gene (for, which encodes a cGMP-dependent protein kinase (PKG)) as well as to its downstream target, protein phosphatase 2A (PP2A). Genetic and pharmacological manipulations revealed that reduced PKG (or PP2A) activity caused increased thermotolerance of synaptic transmission at the larval neuromuscular junction. Like synaptic transmission, feeding movements were preserved at higher temperatures in larvae with lower PKG levels. In a comparative assay, pharmacological manipulations altering thermotolerance in a central circuit of Locusta migratoria demonstrated conservation of this neuroprotective pathway. In this circuit, either the inhibition of PKG or PP2A induced robust thermotolerance of neural function. We suggest that PKG and therefore the polymorphism associated with the allelic variation in for may provide populations with natural variation in heat stress tolerance. for's function in behavior is conserved across most organisms, including ants, bees, nematodes, and mammals. PKG's role in thermotolerance may also apply to these and other species. Natural variation in thermotolerance arising from genes involved in the PKG pathway could impact the evolution of thermotolerance in natural populations. Public Library of Science 2007-08-22 /pmc/articles/PMC1945089/ /pubmed/17712421 http://dx.doi.org/10.1371/journal.pone.0000773 Text en Dawson-Scully et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Dawson-Scully, Ken
Armstrong, Gary A.B.
Kent, Clement
Robertson, R. Meldrum
Sokolowski, Marla B.
Natural Variation in the Thermotolerance of Neural Function and Behavior due to a cGMP-Dependent Protein Kinase
title Natural Variation in the Thermotolerance of Neural Function and Behavior due to a cGMP-Dependent Protein Kinase
title_full Natural Variation in the Thermotolerance of Neural Function and Behavior due to a cGMP-Dependent Protein Kinase
title_fullStr Natural Variation in the Thermotolerance of Neural Function and Behavior due to a cGMP-Dependent Protein Kinase
title_full_unstemmed Natural Variation in the Thermotolerance of Neural Function and Behavior due to a cGMP-Dependent Protein Kinase
title_short Natural Variation in the Thermotolerance of Neural Function and Behavior due to a cGMP-Dependent Protein Kinase
title_sort natural variation in the thermotolerance of neural function and behavior due to a cgmp-dependent protein kinase
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1945089/
https://www.ncbi.nlm.nih.gov/pubmed/17712421
http://dx.doi.org/10.1371/journal.pone.0000773
work_keys_str_mv AT dawsonscullyken naturalvariationinthethermotoleranceofneuralfunctionandbehaviorduetoacgmpdependentproteinkinase
AT armstronggaryab naturalvariationinthethermotoleranceofneuralfunctionandbehaviorduetoacgmpdependentproteinkinase
AT kentclement naturalvariationinthethermotoleranceofneuralfunctionandbehaviorduetoacgmpdependentproteinkinase
AT robertsonrmeldrum naturalvariationinthethermotoleranceofneuralfunctionandbehaviorduetoacgmpdependentproteinkinase
AT sokolowskimarlab naturalvariationinthethermotoleranceofneuralfunctionandbehaviorduetoacgmpdependentproteinkinase