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Evaluation of steroid hormone receptor protein expression in intact cells using flow cytometry
Several methods are currently employed to evaluate expression of steroid hormone receptors in tissues and cells, including real-time reverse-transcriptase polymerase chain reaction (real-time RT-PCR) and western blot assays. These methods require homogenization of cells, thereby preventing evaluatio...
Autores principales: | Butts, Cherie L., Shukair, Shetha A., Duncan, Kristina M., Harris, Christopher W., Belyavskaya, Elena, Sternberg, Esther M. |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Nuclear Receptor Signaling Atlas
2007
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1948072/ https://www.ncbi.nlm.nih.gov/pubmed/17710123 http://dx.doi.org/10.1621/nrs.05007 |
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