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Optimization of biotinyl-tyramide-based in situ hybridization for sensitive background-free applications on formalin-fixed, paraffin-embedded tissue specimens
BACKGROUND: Over the past five years in situ hybridization techniques employing tyramide amplification reagents have been developed and promise the potential detection of low/single-copy nucleic acid sequences. However the increased sensitivity that tyramide amplification brings about may also lead...
| Autores principales: | , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2003
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC194893/ https://www.ncbi.nlm.nih.gov/pubmed/12801424 http://dx.doi.org/10.1186/1472-6890-3-2 |