Discovery of natural nicking endonucleases Nb.BsrDI and Nb.BtsI and engineering of top-strand nicking variants from BsrDI and BtsI

BsrDI and BtsI restriction endonucleases recognize and cleave double-strand DNA at the sequences GCAATG (2/0) and GCAGTG (2/0), respectively. We have purified and partially characterized these two enzymes, and analyzed the genes that encode them. BsrDI and BtsI are unusual in two respects: each clea...

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Detalles Bibliográficos
Autores principales: Xu, Shuang-Yong, Zhu, Zhenyu, Zhang, Penghua, Chan, Siu-Hong, Samuelson, James C., Xiao, Jianping, Ingalls, Debra, Wilson, Geoffrey G.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2007
Materias:
Nucleic Acid Enzymes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1950550/
https://www.ncbi.nlm.nih.gov/pubmed/17586812
http://dx.doi.org/10.1093/nar/gkm481

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1950550/
https://www.ncbi.nlm.nih.gov/pubmed/17586812
http://dx.doi.org/10.1093/nar/gkm481

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